Department of Bioengineering, Graduate School of Kyonggi University, 94-6 Iui-dong, Yeongtong-gu, Suwon 433-760, Republic of Korea.
Basic Science Research Institute, Kyonggi University, 94-6 Iui-dong, Yeongtong-gu, Suwon 443-760, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1108-1115. doi: 10.1099/ijs.0.051078-0. Epub 2013 Dec 20.
A novel aerobic bacterium, designated strain RR11(T), was isolated from peat soil and was characterized by using a polyphasic taxonomic approach and identified in order to determine its taxonomic position. Strain RR11(T) is a Gram-negative, non-sporulating, motile, short-rod-shaped bacterium. 16S rRNA gene sequence analysis identified this strain as a member of the genus Burkholderia of the class Betaproteobacteria. The highest degrees of gene sequence similarity were found with Burkholderia tropica Ppe8(T) (98.0 %), B. bannensis E25(T) (97.3 %), B. ferrariae FeGI01(T) (97.1 %), B. unamae MTI-641(T) (97.1 %) and B. heleia SA41(T) (97.1 %). Strain RR11(T) had the following chemotaxonomic characteristics: the major ubiquinone was Q-8, the DNA G+C content was 60.8 mol%, the major fatty acids were C16 : 0, C19 : 0 cyclo ω8c and C17 : 0 cyclo and the polar lipid profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown aminophospholipid. Based on its morphological, physiological and chemotaxonomic characteristics, together with 16S rRNA gene sequence comparison results, strain RR11(T) represents a novel species, for which the name Burkholderia eburnea sp. nov. is proposed. The type strain is strain RR11(T) ( = KEMC 7302-065(T) = JCM 18070(T)).
一株新的好氧细菌,命名为 RR11(T) 菌株,从泥炭土中分离出来,采用多相分类学方法进行了表征,并进行了鉴定,以确定其分类地位。RR11(T) 菌株是革兰氏阴性、非孢子形成、能动、短杆状细菌。16S rRNA 基因序列分析将该菌株鉴定为β变形菌纲伯克霍尔德氏菌属的成员。与 Burkholderia tropica Ppe8(T)(98.0%)、B. bannensis E25(T)(97.3%)、B. ferrariae FeGI01(T)(97.1%)、B. unamae MTI-641(T)(97.1%)和 B. heleia SA41(T)(97.1%)的基因序列相似度最高。RR11(T) 菌株具有以下化学分类特征:主要的类异戊二烯醌是 Q-8,DNA G+C 含量为 60.8 mol%,主要脂肪酸是 C16:0、C19:0 环 ω8c 和 C17:0 环,极性脂类谱含有磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油和一种未知的氨基磷脂。基于其形态学、生理学和化学分类学特征,以及 16S rRNA 基因序列比较结果,RR11(T) 菌株代表了一个新的种,提议命名为 Burkholderia eburnea sp. nov.。模式菌株为 RR11(T) 菌株(=KEMC 7302-065(T)=JCM 18070(T))。