Gu Jia-Yu, Zang Sheng-Gang, Sheng Xia-Fang, He Lin-Yan, Huang Zhi, Wang Qi
Key Laboratory of Agricultural Environment Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):1031-1037. doi: 10.1099/ijs.0.000059. Epub 2015 Jan 9.
A novel type of mineral-weathering bacterium was isolated from the weathered surface of rock (mica schist) collected from Susong (Anhui, China). Cells of strain L226(T) were Gram-stain-negative. The strain grew optimally at 30 °C, with 1 % (w/v) NaCl and at pH 7.0 in trypticase soy broth. On the basis of 16S rRNA gene phylogeny, strain L226(T) was shown to belong to the genus Burkholderia and the closest phylogenetic relatives were Burkholderia sprentiae WSM5005(T) (98.3 %), Burkholderia acidipaludis NBRC 101816(T) (98.2 %), Burkholderia tuberum STM678(T) (97.2 %) and Burkholderia diazotrophica JPY461(T) (97.1 %). The DNA G+C content was 63.5 mol% and the respiratory quinone was Q-8. The major fatty acids were C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. The polar lipid profile of strain L226(T) consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unknown lipids and unidentified aminophospholipids. Based on the low level of DNA-DNA relatedness (ranging from 25.8 % to 34.4 %) to the tested type strains of species of the genus Burkholderia and unique phenotypic characteristics, it is suggested that strain L226(T) represents a novel species of the genus Burkholderia, for which the name Burkholderia susongensis sp. nov., is proposed. The type strain is L226(T) ( = CCTCC AB2014142(T) = JCM 30231(T)).
从中国安徽宿松采集的岩石（云母片岩）风化表面分离出一种新型矿物风化细菌。菌株L226(T)的细胞革兰氏染色阴性。该菌株在胰蛋白胨大豆肉汤中，于30℃、1%（w/v）NaCl和pH 7.0条件下生长最佳。基于16S rRNA基因系统发育分析，菌株L226(T)被证明属于伯克霍尔德氏菌属，与其亲缘关系最近的系统发育相关菌株为斯普伦氏伯克霍尔德氏菌WSM5005(T)（98.3%）、酸沼伯克霍尔德氏菌NBRC 101816(T)（98.2%）、块茎伯克霍尔德氏菌STM678(T)（97.2%）和固氮伯克霍尔德氏菌JPY461(T)（97.1%）。DNA G+C含量为63.5 mol%，呼吸醌为Q-8。主要脂肪酸为C16 : 0、C17 : 0环丙烷和C19 : 0环丙烷ω8c。菌株L226(T)的极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、未知脂质和未鉴定的氨基磷脂组成。基于与伯克霍尔德氏菌属已测试模式菌株的低水平DNA-DNA相关性（范围为25.8%至34.4%）以及独特的表型特征，表明菌株L226(T)代表伯克霍尔德氏菌属的一个新物种，为此提出新名称宿松伯克霍尔德氏菌（Burkholderia susongensis sp. nov.）。模式菌株为L226(T)（=CCTCC AB2014142(T)=JCM 30231(T)）。
