Tian Di, Ma Xin, Li Yu-E, Zha Liang-Song, Wu Yang, Zou Xiao-Xia, Liu Shuang
College of Territorial Resources and Tourism, Anhui Normal University, Wuhu 241003, China.
Key Laboratory of Agro-Environment and Climate Change, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Huan Jing Ke Xue. 2013 Oct;34(10):4096-104.
Carbon dioxide Capture and Storage has provided a new option for mitigating global anthropogenic CO2 emission with its unique advantages. However, there is a risk of the sealed CO2 leakage, bringing a serious threat to the ecology system. It is widely known that soil microorganisms are closely related to soil health, while the study on the impact of sequestered CO2 leakage on soil microorganisms is quite deficient. In this study, the leakage scenarios of sealed CO2 were constructed and the 16S rRNA genes of soil bacteria were sequenced by Illumina high-throughput sequencing technology on Miseq platform, and related biological analysis was conducted to explore the changes of soil bacterial abundance, diversity and structure. There were 486,645 reads for 43,017 OTUs of 15 soil samples and the results of biological analysis showed that there were differences in the abundance, diversity and community structure of soil bacterial community under different CO, leakage scenarios while the abundance and diversity of the bacterial community declined with the amplification of CO2 leakage quantity and leakage time, and some bacteria species became the dominant bacteria species in the bacteria community, therefore the increase of Acidobacteria species would be a biological indicator for the impact of sealed CO2 leakage on soil ecology system.
二氧化碳捕集与封存以其独特优势为缓解全球人为二氧化碳排放提供了新选择。然而，封存的二氧化碳存在泄漏风险，给生态系统带来严重威胁。众所周知，土壤微生物与土壤健康密切相关，而关于封存二氧化碳泄漏对土壤微生物影响的研究相当匮乏。本研究构建了封存二氧化碳的泄漏场景，利用Illumina高通量测序技术在Miseq平台上对土壤细菌的16S rRNA基因进行测序，并进行相关生物学分析，以探究土壤细菌丰度、多样性和结构的变化。15个土壤样本的43017个操作分类单元（OTU）共获得486645条序列读数，生物学分析结果表明，不同二氧化碳泄漏场景下土壤细菌群落的丰度、多样性和群落结构存在差异，细菌群落的丰度和多样性随二氧化碳泄漏量和泄漏时间的增加而下降，一些细菌种类成为细菌群落中的优势菌种，因此酸杆菌种类的增加将是封存二氧化碳泄漏对土壤生态系统影响的一个生物学指标。
