Wu Qin, Chen Lei, Wang Jiangxin, Zhang Weiwen
Sheng Wu Gong Cheng Xue Bao. 2013 Aug;29(8):1086-99.
Photosynthetic cyanobacteria possess a series of good properties, such as their abilities to capture solar energy for CO2 fixation, low nutritional requirements for growth, high growth rate, and relatively simple genetic background. Due to the high oil price and increased concern of the global warming in recent years, cyanobacteria have attracted widespread attention because they can serve as an 'autotrophic microbial factory' for producing renewable biofuels and fine chemicals directly from CO2. Particularly, significant progress has been made in applying synthetic biology techniques and strategies to construct and optimize cyanobacteria chassis. In this article, we critically summarized recent advances in developing new methods to optimize cyanobacteria chassis, improving cyanobacteria photosynthetic efficiency, and in constructing cyanobacteria chassis tolerant to products or environmental stresses. In addition, various industrial applications of cyanobacteria chassis are also discussed.
光合蓝细菌具有一系列优良特性,例如它们能够捕获太阳能以固定二氧化碳、生长所需营养需求低、生长速率高以及遗传背景相对简单。由于近年来油价高企以及全球变暖问题日益受到关注,蓝细菌因其可作为“自养微生物工厂”直接从二氧化碳生产可再生生物燃料和精细化学品而备受广泛关注。特别是,在应用合成生物学技术和策略构建和优化蓝细菌底盘方面取得了显著进展。在本文中,我们批判性地总结了在开发优化蓝细菌底盘的新方法、提高蓝细菌光合效率以及构建对产物或环境胁迫具有耐受性的蓝细菌底盘方面的最新进展。此外,还讨论了蓝细菌底盘的各种工业应用。