Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China.
Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China.
Biotechnol Adv. 2014 Mar-Apr;32(2):541-8. doi: 10.1016/j.biotechadv.2014.02.001. Epub 2014 Feb 13.
Large-scale production of renewable biofuels through microbiological processes has drawn significant attention in recent years, mostly due to the increasing concerns on the petroleum fuel shortages and the environmental consequences of the over-utilization of petroleum-based fuels. In addition to native biofuel-producing microbes that have been employed for biofuel production for decades, recent advances in metabolic engineering and synthetic biology have made it possible to produce biofuels in several non-native biofuel-producing microorganisms. Compared to native producers, these non-native systems carry the advantages of fast growth, simple nutrient requirements, readiness for genetic modifications, and even the capability to assimilate CO2 and solar energy, making them competitive alternative systems to further decrease the biofuel production cost. However, the tolerance of these non-native microorganisms to toxic biofuels is naturally low, which has restricted the potentials of their application for high-efficiency biofuel production. To address the issues, researches have been recently conducted to explore the biofuel tolerance mechanisms and to construct robust high-tolerance strains for non-native biofuel-producing microorganisms. In this review, we critically summarize the recent progress in this area, focusing on three popular non-native biofuel-producing systems, i.e. Escherichia coli, Lactobacillus and photosynthetic cyanobacteria.
近年来，通过微生物过程大规模生产可再生生物燃料引起了人们的极大关注，主要是因为人们对石油燃料短缺的担忧日益增加，以及过度利用石油基燃料所带来的环境后果。除了几十年来一直用于生物燃料生产的本土生物燃料生产微生物外，代谢工程和合成生物学的最新进展使得在几种非本土生物燃料生产微生物中生产生物燃料成为可能。与本土生产者相比，这些非本土系统具有生长速度快、营养需求简单、易于基因改造甚至能够同化 CO2 和太阳能等优势，使它们成为有竞争力的替代系统，可以进一步降低生物燃料的生产成本。然而，这些非本土微生物对有毒生物燃料的耐受性自然较低，这限制了它们在高效生物燃料生产中的应用潜力。为了解决这些问题，最近的研究探索了生物燃料耐受性机制，并为非本土生物燃料生产微生物构建了具有强大耐受性的菌株。在这篇综述中，我们批判性地总结了这一领域的最新进展，重点介绍了三种流行的非本土生物燃料生产系统，即大肠杆菌、乳杆菌和光合蓝藻。
