Li Lei, Lu Yinhua, Jiang Weihong
Sheng Wu Gong Cheng Xue Bao. 2013 Aug;29(8):1113-22.
In 2010, the artificial synthesis of Mycoplasma mycoides triggers the new era of synthetic biology. This great breakthrough is achieved mainly thanks to the powerful DNA recombinant ability of yeast. In recent years, except for the methods used for large DNA assembly on the basis of in vivo homologous recombination, various different DNA assembly methods in vitro, based on the concept of DNA ligation or polymerization, have also been developed, such as Biobrick\BglBrick, SLIC and Gibson one-step assembly. Application of these new technologies has greatly accelerated the construction of synthetic part libraries, biosynthetic pathway and even microbial chromosomes. In fact, all DNA assembly methods are derived from the combinations of DNA joining and organizational schemes. This review describes the brief introduction of the main in vivo and in vitro DNA assembly protocols developed so for, which will benefit the construction of different types of synthetic functional devices and also biosynthetic pathways in the research of synthetic biology in China.
2010年，人工合成丝状支原体开启了合成生物学的新纪元。这一重大突破的实现主要归功于酵母强大的DNA重组能力。近年来，除了基于体内同源重组的大型DNA组装方法外，还开发了各种基于DNA连接或聚合概念的体外不同DNA组装方法，如Biobrick\BglBrick、SLIC和吉布森一步组装法。这些新技术的应用极大地加速了合成部件库、生物合成途径乃至微生物染色体的构建。实际上，所有DNA组装方法都源自DNA连接和组织方案的组合。本文综述了迄今为止开发的主要体内和体外DNA组装方案，这将有助于中国合成生物学研究中不同类型合成功能装置以及生物合成途径的构建。
