构建复杂合成电路的模块化方法。

A Modular Approach to Building Complex Synthetic Circuits.

作者信息

Li Yinqing, Weiss Ron

机构信息

Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massacheusetts Ave., Cambridge, MA, 02139, USA.

Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massacheusetts Ave., Cambridge, MA, 02139, USA.

出版信息

Methods Mol Biol. 2017;1651:231-248. doi: 10.1007/978-1-4939-7223-4_17.

DOI:10.1007/978-1-4939-7223-4_17

PMID:28801911

Abstract

Efficient assembly of genetic circuits is a critical element in synthetic biology for implementing complex gene expression systems. Here we present an assembly framework that integrates recent developments in DNA recombination technologies and unique nucleotide sequences for modular and reliable construction of complex genetic circuits. All relevant protocols are described in detail. Implementing these protocols relies on readily available reagents and basic molecular biology techniques without the need for specialized equipment.

摘要

遗传电路的高效组装是合成生物学中实现复杂基因表达系统的关键要素。在此，我们提出了一个组装框架，该框架整合了DNA重组技术的最新进展以及独特的核苷酸序列，用于模块化且可靠地构建复杂的遗传电路。所有相关方案都进行了详细描述。实施这些方案依赖于易于获得的试剂和基本的分子生物学技术，无需专门设备。