Wójcik-Fatla Angelina, Zając Violetta, Sroka Jacek, Piskorski Michał, Cisak Ewa, Sawczyn Anna, Dutkiewicz Jacek
Department of Zoonoses, Institute of Rural Health, Lublin, Poland.
Department of Zoonoses, Institute of Rural Health, Lublin, Poland; Department of Parasitology, National Veterinary Research Institute, Puławy, Poland.
Ann Agric Environ Med. 2013;20(4):705-7.
Samples of 30 dead small mammals each were collected on area 'A' located in eastern Poland and exposed to floods by the Vistula river, and on area 'B', also located in eastern Poland, but not exposed to floods. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of Leptospira DNA. From 7 species of small mammals examined, the presence of Leptospira DNA was detected in 2 of them. The prevalence of positive results was greatest in Apodemus agrarius which was the mostly numerous mammal species (14 out of total 39 specimens, 35.9%). The presence of Leptospira DNA was also found in Microtus arvalis (1 out of 1 specimen, 100%), whereas the remaining 5 species (Apodemus flavicollis , Apodemus sylvaticus, Microtus agrestis, Myodes glareolus, Sorex araneus) were negative. No significant difference in the prevalence of positive findings was found between the small mammals from areas 'A' exposed to flooding, compared to those from area 'B' not exposed to flooding (20.0% vs. 30.0%, p=0.3748). The overall positivity of the examined small mammals population from areas 'A' and 'B' was 25.0%. The prevalence of dual positivity (leptospiral DNA found both in kidney and liver) was greater in the mammals from areas exposed to flooding compared to those from areas not exposed to flooding (16.7% vs. 6.7%), but this dependence was also not significant (p=0.2382).
在波兰东部受维斯瓦河洪水影响的“A”区域和同样位于波兰东部但未受洪水影响的“B”区域，分别采集了30只死亡小型哺乳动物的样本。通过聚合酶链反应（PCR）和巢式聚合酶链反应方法检测这些哺乳动物的肾脏和肝脏中是否存在钩端螺旋体DNA。在所检测的7种小型哺乳动物中，有2种检测到钩端螺旋体DNA。阳性结果的患病率在黑线姬鼠中最高，它是数量最多的哺乳动物物种（39个样本中有14个，占35.9%）。在田鼠中也发现了钩端螺旋体DNA（1个样本中有1个，占100%），而其余5种（黄颈姬鼠、林姬鼠、草原田鼠、棕背䶄、普通刺猬）均为阴性。与未受洪水影响的“B”区域的小型哺乳动物相比，受洪水影响的“A”区域的小型哺乳动物阳性结果的患病率没有显著差异（20.0%对30.0%，p = 0.3748）。来自“A”区域和“B”区域的受检小型哺乳动物总体阳性率为25.0%。与未受洪水影响区域的哺乳动物相比，受洪水影响区域的哺乳动物双重阳性（肾脏和肝脏中均发现钩端螺旋体DNA）的患病率更高（16.7%对6.7%），但这种相关性也不显著（p = 0.2382）。
