Niemcewicz Marcin, Pająk Beata, Michalski Aleksander, Kocik Janusz, Kołodziej Marcin, Joniec Justyna, Graniak Grzegorz, Gaweł Jerzy, Marciniak-Niemcewicz Anna, Kucharczyk Krzysztof, Prystupa Andrzej, Witczak Agnieszka, Lasocki Krzysztof, Naylor Katarzyna, Goniewicz Mariusz
Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
BioVectis, Warsaw, Poland; Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
Ann Agric Environ Med. 2013;20(4):711-4.
Influenza is a contagious respiratory disease caused by viruses belonging to the family Ortomyxoviridae. Among the influenza viruses type A, B and C, the A type virus shows the most pathogenic potential. Its surface receptor glycoproteins, hemagglutinin (HA) and neuraminidase (NA), are characterized by high antigenic variation, thus a host organism cannot develop permanent resistance. The case is described of a male patient with severe acute respiratory distress syndrome in the course of influenza A/N1H1v infection, confirmed by virological molecular analysis. During diagnostic procedures based on the MSSCP genotyping it was observed that the WHO recommended RT-PCR kits and/or procedure of sample collection from patients for molecular investigation could lead to false positive A/H1N1 pandemic strain detection because of the co-amplification during the RT-PCR fragments of the human genome.
