Kasperczyk Sławomir, Błaszczyk Iwona, Dobrakowski Michał, Romuk Ewa, Kapka-Skrzypczak Lucyna, Adamek Mariusz, Birkner Ewa
Department of Biochemistry, Medical University of Silesia, Katowice, Poland.
Independent Laboratory of Molecular Biology, Institute of Rural Health, Lublin, Poland; Department of Public Health, University of Information Technology and Management, Rzeszow, Poland.
Ann Agric Environ Med. 2013;20(4):721-5.
The most important biothiols include glutathione, homocysteine (HCY), cysteine and proteins. The aim of the presented study was to evaluate the influence of lead on the biothiol turnover--the concentration of HCY and protein sulfhydryl groups (P-SH) in the serum and reduced glutathione (G-SH) in erythrocytes--in individuals (employees of metal works) exposed to lead and to evaluate its probable oxidative disorders, measured as the carbonyl protein (CP) concentration in serum. The exposed workers were divided into 2 subgroups: 1) low lead exposure (LPb), with a lead concentration in the blood (PbB) of 20-45 µg dl(-1) (n= 102), and 2) high lead exposure (HPb), with PbB = 45-60 µg dl(-1) (n= 81). The control group consisted of 72 office workers or other healthy subjects with no history of occupational exposure to lead. All the controls had normal PbB (<10 μg dl(-1)) and ZPP (<2.5 μg dl(-1)) levels. The concentration of HCY was higher in the LPb group by 11% and in the HPb group by 26%, compared with the control group (n=72). The CP concentration in these 2 groups was more than twice as high as that of the control group, with 108% and 125% increases for the LPb and HPb groups, respectively; G-SH was lower by 6.6% and 7.4% for the LPb and HPb groups, respectively; P-SH was lower by 8.2% and 13% for the LPb and HPb groups, respectively. Lead decreases levels of glutathione and protein thiol groups. Lead-induced oxidative stress contributes to the observed elevation of protein carbonyl groups. Besides, lead poisoning seems to be associated with hyperhomocysteinaemia, which may promote the development of atherosclerosis.
最重要的生物硫醇包括谷胱甘肽、同型半胱氨酸（HCY）、半胱氨酸和蛋白质。本研究的目的是评估铅对生物硫醇周转的影响——血清中HCY和蛋白质巯基（P-SH）的浓度以及红细胞中还原型谷胱甘肽（G-SH）的浓度——在接触铅的个体（金属工厂工人）中，并评估其可能的氧化紊乱情况，以血清中羰基蛋白（CP）浓度来衡量。接触铅的工人被分为2个亚组：1）低铅暴露组（LPb），血铅浓度（PbB）为20 - 45 μg dl⁻¹（n = 102），2）高铅暴露组（HPb），PbB = 45 - 60 μg dl⁻¹（n = 81）。对照组由72名办公室工作人员或其他无职业铅暴露史的健康受试者组成。所有对照组的PbB（<10 μg dl⁻¹）和ZPP（<2.5 μg dl⁻¹）水平均正常。与对照组（n = 72）相比，LPb组的HCY浓度高11%，HPb组高26%。这两组的CP浓度比对照组高出两倍多，LPb组和HPb组分别增加了108%和125%；LPb组和HPb组的G-SH分别降低了6.6%和7.4%；LPb组和HPb组的P-SH分别降低了8.2%和13%。铅会降低谷胱甘肽和蛋白质硫醇基团的水平。铅诱导的氧化应激导致了观察到的蛋白质羰基基团升高。此外，铅中毒似乎与高同型半胱氨酸血症有关，这可能会促进动脉粥样硬化的发展。
