无机磷酸盐对体外葡萄糖诱导的胰岛素释放的影响。

Influence of inorganic phosphate on glucose-induced insulin release in vitro.

作者信息

Osuna J I, Castillo M, Rodriguez E, Campillo J E, Osorio C

出版信息

Adv Exp Med Biol. 1986;208:509-15. doi: 10.1007/978-1-4684-5206-8_63.

Abstract

The effect of various extracellular phosphate concentrations on glucose induced insulin secretion has been investigated in two experimental systems the isolated perfused rat pancreas and the isolated rat pancreatic islets incubated in vitro. In both systems, total insulin secretion in response to 16.7 mmol/l glucose increased as extracellular phosphate concentration increased from 0 to 3.6 mmol/l. In perfusion experiments insulin secretion was significantly reduced at 4.8 mmol/l phosphate. In incubation experiments insulin secretion was inhibited only at 9.6 mmol/l phosphate concentration. When IBMX 1 mmol/l was used in incubation experiments glucose induced insulin secretion increased as extracellular phosphate increased from 0 to 3.6 mmol/l, but was also inhibited at 4.8 mmol/l.

摘要

在两个实验系统中研究了不同细胞外磷酸盐浓度对葡萄糖诱导的胰岛素分泌的影响，这两个系统分别是离体灌注大鼠胰腺和体外培养的离体大鼠胰岛。在这两个系统中，随着细胞外磷酸盐浓度从0增加到3.6 mmol/l，对16.7 mmol/l葡萄糖的总胰岛素分泌量增加。在灌注实验中，磷酸盐浓度为4.8 mmol/l时胰岛素分泌显著减少。在培养实验中，仅在磷酸盐浓度为9.6 mmol/l时胰岛素分泌受到抑制。当在培养实验中使用1 mmol/l的异丁基甲基黄嘌呤（IBMX）时，随着细胞外磷酸盐从0增加到3.6 mmol/l，葡萄糖诱导的胰岛素分泌增加，但在4.8 mmol/l时也受到抑制。