Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China.
Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China.
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1496-502. doi: 10.1016/j.msec.2012.04.031. Epub 2012 Apr 28.
A biomimetic nanofibrous poly(L-lactide) scaffold decorated by chitosan nanofiber network inside the macropores was fabricated using a dual thermally induced phase separation technique. The first phase separation was used to build a nanofibrous poly(L-lactide) scaffold with interconnected macropores, where chitosan nanofibers about 500nm in diameter were incorporated via the second phase separation. The content of nanofibrous chitosan was determined to be 5.76 in weight percentage by elemental analysis. The composite scaffold showed the highest protein adsorption of 7225±116 μg/cm(3) and the most hydroxyapatite crystal deposition in the mineralization. Compared with non-nanofibrous poly(L-lactide) scaffold, nanofibrous poly(L-lactide) scaffold exhibited a much faster degradation, but it could be restrained by the introduced chitosan nanofibers. The bone mesenchymal stem cell culture results indicated that the cells would rather attach and stretch along the chitosan nanofibers in the composite scaffold that showed the highest viability and the best cytocompatibility may be attributed to the biomimetic nanofibrous network and good cell affinity of chitosan nanofibers.
采用双重热致相分离技术制备了一种具有仿生纳米纤维网络的壳聚糖修饰聚乳酸（PLLA）多孔支架。该技术首先通过相分离作用制备出具有相互贯通的大孔的纳米纤维 PLLA 支架，然后通过第二次相分离作用将直径约 500nm 的壳聚糖纳米纤维引入其中。元素分析表明，纳米纤维壳聚糖的含量为 5.76wt%。该复合支架的蛋白质吸附量最高，达到 7225±116μg/cm(3)，矿化过程中羟基磷灰石晶体的沉积量最多。与非纳米纤维 PLLA 支架相比，纳米纤维 PLLA 支架的降解速度更快，但引入壳聚糖纳米纤维后可以对其进行抑制。骨髓间充质干细胞培养结果表明，细胞更喜欢在复合支架中沿着壳聚糖纳米纤维黏附和伸展，复合支架的细胞活力最高，细胞相容性最好，这可能归因于仿生纳米纤维网络和壳聚糖纳米纤维良好的细胞亲和性。
