Ma Wei, Zhang Sihan, Xie Chong, Wan Xing, Li Xiaofeng, Chen Kebing, Zhao Guanglei
State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China.
Department of Spine Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, 26 Erheng Road, Yuan Village, Guangzhou, 510655, China.
Polymers (Basel). 2022 May 20;14(10):2083. doi: 10.3390/polym14102083.
The majority of chitosan-based bone tissue engineering (BTE) scaffolds have the problem of poor mechanical properties. However, modifying chitosan with conventional silane coupling agents to improve the mechanical properties of scaffolds will introduce additional complications, including cytotoxicity and poor biocompatibility. In this study, two types of organic−inorganic composite scaffolds (F-A-T0/T3/T5 and F-B-T5-P0/P0.5/P1.5/P2.5) were prepared using chitosan nanofibers (CSNF) prepared by the beating-homogenization method, combined with the sol−gel method, and further introduced polyvinyl alcohol (PVA). The F-A-T3 and F-B-T5-P1.5 exhibited interconnected pore and surface nanofibers structures, high porosity (>70%), outstanding swelling properties, and a controllable degradation rate. The Young’s modulus of TEOS: 5.0% (w/w), PVA: 1.5% (w/w) chitosan fiber scaffold is 8.53 ± 0.43 MPa in dry conditions, and 237.78 ± 8.86 kPa in wet conditions, which is four times that of F-A-T5 and twice that of F-B-T5-P0. Additionally, cell (MC3T3-E1) experiments confirmed that the two composite scaffolds had great cytocompatibility and were predicted to be used in the future in the field of BTE scaffolds.
大多数基于壳聚糖的骨组织工程（BTE）支架存在机械性能差的问题。然而，用传统的硅烷偶联剂对壳聚糖进行改性以改善支架的机械性能会带来额外的复杂性，包括细胞毒性和生物相容性差。在本研究中，使用通过打浆-均质法制备的壳聚糖纳米纤维（CSNF），结合溶胶-凝胶法，并进一步引入聚乙烯醇（PVA），制备了两种类型的有机-无机复合支架（F-A-T0/T3/T5和F-B-T5-P0/P0.5/P1.5/P2.5）。F-A-T3和F-B-T5-P1.5呈现出相互连通的孔隙和表面纳米纤维结构、高孔隙率（>70%）、出色的溶胀性能以及可控的降解速率。在干燥条件下，TEOS: 5.0%（w/w）、PVA: 1.5%（w/w）的壳聚糖纤维支架的杨氏模量为8.53±0.43 MPa，在潮湿条件下为237.78±8.86 kPa，分别是F-A-T5的四倍和F-B-T5-P0的两倍。此外，细胞（MC3T3-E1）实验证实这两种复合支架具有良好的细胞相容性，预计未来可用于BTE支架领域。
