基于通过将葡萄糖氧化酶吸附到核壳型 Fe3O4@SiO2@Au 磁性纳米粒子上来增强对氧的催化还原作用的安培型葡萄糖传感器。
Amperometric glucose sensor based on enhanced catalytic reduction of oxygen using glucose oxidase adsorbed onto core-shell Fe3O4@silica@Au magnetic nanoparticles.
机构信息
College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; Key Laboratory of Green Chemical Media and Reactions, Ministry of Education; School of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China.
College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China.
出版信息
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1640-7. doi: 10.1016/j.msec.2012.04.055. Epub 2012 Apr 29.
Monodisperse Fe3O4 magnetic nanoparticles (NPs) were prepared under facile solvothermal conditions and successively functionalized with silica and Au to form core/shell Fe3O4@silica@Au NPs. Furthermore, the samples were used as matrix to construct a glucose sensor based on glucose oxidase (GOD). The immobilized GOD retained its bioactivity with high protein load of 3.92×10(-9) mol·cm(-2), and exhibited a surface-controlled quasi-reversible redox reaction, with a fast heterogeneous electron transfer rate of 7.98±0.6 s(-1). The glucose biosensor showed a broad linear range up to 3.97 mM with high sensitivity of 62.45 μA·mM(-1) cm(-2) and fast response (less than 5s).
单分散四氧化三铁磁性纳米粒子（NPs）在简单的溶剂热条件下制备，并依次用硅和 Au 进行功能化，形成核/壳 Fe3O4@silica@Au NPs。此外，这些样品被用作基质来构建基于葡萄糖氧化酶（GOD）的葡萄糖传感器。固定化的 GOD 保留了高蛋白质负载（3.92×10(-9) mol·cm(-2)）的生物活性，表现出表面控制的准可逆氧化还原反应，具有 7.98±0.6 s(-1)的快速非均相电子转移速率。葡萄糖生物传感器具有宽线性范围，高达 3.97 mM，具有高灵敏度（62.45 μA·mM(-1) cm(-2)）和快速响应（小于 5s）。
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