Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan.
Graduate School of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan.
Sensors (Basel). 2020 Feb 11;20(4):964. doi: 10.3390/s20040964.
In this study, the potentiometric arrayed glucose biosensors, which were based on zinc oxide (ZnO) or aluminum-doped zinc oxide (AZO) sensing membranes, were fabricated by using screen-printing technology and a sputtering system, and graphene oxide (GO) and Nafion-glucose oxidase (GOx) were used to modify sensing membranes by using the drop-coating method. Next, the material properties were characterized by using a Raman spectrometer, a field-emission scanning electron microscope (FE-SEM), and a scanning probe microscope (SPM). The sensing characteristics of the glucose biosensors were measured by using the voltage-time (V-T) measurement system. Finally, electrochemical impedance spectroscopy (EIS) was conducted to analyze their charge transfer abilities. The results indicated that the average sensitivity of the glucose biosensor based on Nafion-GOx/GO/AZO was apparently higher than that of the glucose biosensor based on Nafion-GOx/GO/ZnO. In addition, the glucose biosensor based on Nafion-GOx/GO/AZO exhibited an excellent average sensitivity of 15.44 mV/mM and linearity of 0.997 over a narrow range of glucose concentration range, a response time of 26 s, a limit of detection (LOD) of 1.89 mM, and good reproducibility. In terms of the reversibility and stability, the hysteresis voltages (V) were 3.96 mV and 2.42 mV. Additionally, the glucose biosensor also showed good anti-inference ability and reproducibility. According to these results, it is demonstrated that AZO is a promising material, which could be used to develop a reliable, simple, and low-cost potentiometric glucose biosensor.
在这项研究中，我们采用丝网印刷技术和溅射系统制备了基于氧化锌（ZnO）或铝掺杂氧化锌（AZO）传感膜的电位阵列葡萄糖生物传感器，并采用滴涂法将氧化石墨烯（GO）和Nafion-葡萄糖氧化酶（GOx）修饰在传感膜上。接下来，我们使用拉曼光谱仪、场发射扫描电子显微镜（FE-SEM）和扫描探针显微镜（SPM）对材料特性进行了表征。我们使用电压-时间（V-T）测量系统测量了葡萄糖生物传感器的传感特性。最后，我们通过电化学阻抗谱（EIS）分析了它们的电荷转移能力。结果表明，基于 Nafion-GOx/GO/AZO 的葡萄糖生物传感器的平均灵敏度明显高于基于 Nafion-GOx/GO/ZnO 的葡萄糖生物传感器。此外，基于 Nafion-GOx/GO/AZO 的葡萄糖生物传感器在较窄的葡萄糖浓度范围内表现出出色的平均灵敏度为 15.44 mV/mM 和线性度为 0.997，响应时间为 26 s，检测限（LOD）为 1.89 mM，具有良好的重现性。在可逆性和稳定性方面，滞后电压（V）分别为 3.96 mV 和 2.42 mV。此外，该葡萄糖生物传感器还表现出良好的抗干扰能力和重现性。根据这些结果，表明 AZO 是一种很有前途的材料，可用于开发可靠、简单且低成本的电位型葡萄糖生物传感器。
