基于金@硅核壳纳米粒子的表面增强拉曼散射生物传感器用于无标记葡萄糖检测。
A gold@silica core-shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection.
机构信息
Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq; Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA.
Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA.
出版信息
Anal Chim Acta. 2014 Feb 6;811:76-80. doi: 10.1016/j.aca.2013.12.009. Epub 2014 Jan 3.
The gold nanostar@silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25 μM to 25 mM in the aqueous solution with the limit of detection of 16 μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample.
金纳米星@二氧化硅核壳纳米粒子与葡萄糖氧化酶（GOx）酶分子结合，已被开发为用于无标记检测葡萄糖的表面增强拉曼散射（SERS）生物传感器。表面固定化的 GOx 酶催化葡萄糖的氧化，产生过氧化氢。在激光激发下，Au 纳米星@二氧化硅纳米粒子附近产生的 H2O2 分子产生强 SERS 信号，用于测量葡萄糖浓度。基于纳米星@二氧化硅∼GOx 纳米粒子的传感分析的 SERS 信号显示出对水溶液中葡萄糖浓度范围从 25 μM 到 25 mM 的动态响应，检测限为 16 μM。当葡萄糖与抗坏血酸和尿酸共存时，传感分析没有显示出任何干扰。该传感器可应用于唾液样本。
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