不同修饰 BEA-沸石对电导型生物传感器操作特性的影响。
Effect of different modifications of BEA-zeolites on operational characteristics of conductometric biosensor.
机构信息
Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine; Institute of Biology, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.
Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine.
出版信息
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1648-53. doi: 10.1016/j.msec.2012.04.056. Epub 2012 Apr 28.
Effect of different modifications of zeolite Na(+)-BEA on working characteristics of urease-based conductometric biosensor was studied. As the biosensor sensitive elements were used bioselective membranes based on urease and various zeolites immobilised with bovine serum albumin on the surface of conductometric transducers. Influence of zeolites on sensitivity of urea biosensor was investigated as well as reproducibility of biosensor signal and reproducibility of activity of the bioselective element after different variants of urease immobilisation on the surface of conductometric transducer. The biosensors based on zeolites (NH4(+)-BEA 30 and H(+)-BEA 30) were shown to be the most sensitive. Concentration of these zeolites in the bioselective membrane was optimized. Use of zeolites modified with methyl viologen and silver was ascertained to be of no prospect for urea conductometric biosensors. It was demonstrated that characteristics of urea biosensors can be regulated, varying zeolites modifications and their concentrations in bioselective membranes.
研究了沸石 Na(+)-BEA 的不同修饰方式对基于脲酶的电导生物传感器工作特性的影响。作为生物传感器敏感元件，使用了基于脲酶和各种沸石的生物选择性膜，这些沸石通过牛血清白蛋白固定在电导传感器的表面。研究了沸石对尿素生物传感器灵敏度的影响，以及在不同的脲酶固定在电导传感器表面的变体后，生物选择性元件的信号重现性和活性重现性。基于沸石（NH4(+)-BEA 30 和 H(+)-BEA 30）的生物传感器被证明是最敏感的。优化了生物选择性膜中这些沸石的浓度。确定了用甲紫和银修饰的沸石在尿素电导生物传感器中没有应用前景。结果表明，可以通过改变生物选择性膜中沸石的修饰方式和浓度来调节尿素生物传感器的特性。
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