Zhengzhou University, Henan, China.
University of Wisconsin-Madison, Wisconsin, USA.
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1674-81. doi: 10.1016/j.msec.2012.04.064. Epub 2012 Apr 29.
In this study, poly(ε-caprolactone) (PCL)/sodium chloride (NaCl), PCL/poly(ethylene oxide) (PEO)/NaCl and PCL/PEO/NaCl/hydroxyapatite (HA) composites were injection molded and characterized. The water soluble and sacrificial polymer, PEO, and NaCl particulates in the composites were leached by deionized water to produce porous and interconnected microstructures. The effect of leaching time on porosity, and residual contents of NaCl and NaCl/HA, as well as the effect of HA addition on mechanical properties was investigated. In addition, the biocompatibility was observed via seeding human mesenchymal stem cells (hMSCs) on PCL and PCL/HA scaffolds. The results showed that the leaching time depends on the spatial distribution of sacrificial PEO phase and NaCl particulates. The addition of HA has significantly improved the elastic (E') and loss moduli (E″) of PCL/HA scaffolds. Human MSCs were observed to have attached and proliferated on both PCL and PCL/HA scaffolds. Taken together, the molded PCL and PCL/HA scaffolds could be good candidates as tissue engineering scaffolds. Additionally, injection molding would be a potential and high throughput technology to fabricate tissue scaffolds.
在这项研究中，聚己内酯（PCL）/氯化钠（NaCl）、PCL/聚氧化乙烯（PEO）/NaCl 和 PCL/PEO/NaCl/羟基磷灰石（HA）复合材料被注塑成型并进行了表征。复合材料中的水溶性和牺牲性聚合物 PEO 和 NaCl 颗粒通过去离子水浸出，产生多孔且相互连通的微观结构。研究了浸出时间对多孔性、NaCl 和 NaCl/HA 的残留含量的影响，以及 HA 添加剂对机械性能的影响。此外，通过在 PCL 和 PCL/HA 支架上接种人骨髓间充质干细胞（hMSCs）来观察其生物相容性。结果表明，浸出时间取决于牺牲性 PEO 相和 NaCl 颗粒的空间分布。HA 的添加显著提高了 PCL/HA 支架的弹性（E'）和损耗模量（E″）。在 PCL 和 PCL/HA 支架上均观察到 hMSCs 附着和增殖。总之，注塑成型的 PCL 和 PCL/HA 支架可以作为组织工程支架的良好候选材料。此外，注塑成型可能是一种潜在的高通量制造组织支架的技术。
