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[猪肾Na⁺,K⁺-ATP酶β亚基的一级结构。I. 固定化蛋白的胰蛋白酶水解产物分析]

[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. I. Analysis of products of trypsin hydrolysis of immobilized proteins].

作者信息

Arzamazova N M, Gevondian N M, Chertova E N, Nazimov I V, Gavril'eva E E

出版信息

Bioorg Khim. 1987 Jan;13(1):5-13.

PMID:3032209
Abstract

The Na+, K+-ATPase's beta-subunit immobilized on thiol-glass was hydrolyzed with trypsin. Over 25 peptides covering ca. 90% of the protein polypeptide chain were isolated from the digest by HPLC and characterized. Structural analysis allowed us to localize the sites of attachment of all three carbohydrate chains of beta-subunit. Sequence data were used to design of oligonucleotide hybridization probes for gene cloning.

摘要

固定在硫醇玻璃上的钠钾ATP酶β亚基用胰蛋白酶水解。通过高效液相色谱法从消化产物中分离出25种以上覆盖约90%蛋白质多肽链的肽段并进行了表征。结构分析使我们能够定位β亚基所有三条糖链的连接位点。序列数据用于设计基因克隆的寡核苷酸杂交探针。

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