Holden S A, Teicher B A, Boeheim K, Weichselbaum R R, Ervin T J
Br J Cancer. 1987 Mar;55(3):245-8. doi: 10.1038/bjc.1987.47.
The SCC-25 cell line is a well-established line derived from a human squamous carcinoma of the head and neck. The capacity of this cell line for recovery from potentially lethal damage following X-ray treatment has been documented. The survival curve of stationary phase SCC-25 cells exposed to various concentrations of bleomycin is biphasic with an initial sensitive phase and a less sensitive second phase as is common for many cell lines. Stationary phase SCC-25 cells were exposed to 100 mU ml-1 of bleomycin for 1 h. The drug was removed and the cells were allowed various periods to recover from potentially lethal damage. After 24 h, the SCC-25 cells showed a recovery ratio (R/R0) of 7.0 which corresponded to an immediate survival at a drug level of 27 mU ml-1, a dose 3.7-fold less than the exposure concentration of 100 mU ml-1. Over the course of the first 4 h following bleomycin exposure, 0.5 microM CDDP was a very effective inhibitor of potentially lethal damage repair, giving a R/R0 of 1.1 or nearly complete inhibition of recovery. Between 2 and 4 h the R/R0 was 1.6-1.8 with CDDP and 4.1-5.3 without CDDP indicating appreciable inhibition of recovery. Plant (10 microM) and Plato (10 microM) produced potentially lethal damage recovery inhibition patterns very similar to that of CDDP. After 1 h the recovery ratios in the presence of Plant and Plato were 1.1-1.3. Between 2 and 4 h, Plato and Plant gave recovery ratios of 1.8-2.3 and 1.6-1.9, respectively. NIPt and Pt(terpy) were examined at both 10 microM and 25 microM for their ability to inhibit potentially lethal damage recovery after bleomycin treatment. After 1 h, NIPt gave a recovery ratio of 1.3-1.4, and after 2-4 h the recovery ratio was 1.7-2.6. Pt(terpy) gave recovery ratios of 1.3-1.6 after 1 h and 1.5-1.8 after 24 h.
SCC - 25细胞系是一种源自人头部和颈部鳞状癌的成熟细胞系。该细胞系在X射线处理后从潜在致死性损伤中恢复的能力已有文献记载。处于静止期的SCC - 25细胞暴露于不同浓度博来霉素后的存活曲线呈双相，具有初始敏感阶段和较不敏感的第二阶段，这对许多细胞系来说是常见的。将处于静止期的SCC - 25细胞暴露于100 mU/ml的博来霉素中1小时。去除药物后，让细胞在不同时间段从潜在致死性损伤中恢复。24小时后，SCC - 25细胞的恢复率（R/R0）为7.0，这相当于在药物水平为27 mU/ml时的即时存活率，该剂量比100 mU/ml的暴露浓度低3.7倍。在博来霉素暴露后的前4小时内，0.5 microM顺铂是潜在致死性损伤修复的非常有效的抑制剂，恢复率（R/R0）为1.1，即几乎完全抑制恢复。在2至4小时之间，有顺铂时R/R0为1.6 - 1.8，无顺铂时为4.1 - 5.3，表明恢复受到明显抑制。普兰（10 microM）和普拉托（10 microM）产生的潜在致死性损伤恢复抑制模式与顺铂非常相似。1小时后，存在普兰和普拉托时的恢复率为1.1 - 1.3。在2至4小时之间，普拉托和普兰的恢复率分别为1.8 - 2.3和1.6 - 1.9。研究了10 microM和25 microM的NIPt和Pt(terpy)在博来霉素处理后抑制潜在致死性损伤恢复的能力。1小时后，NIPt的恢复率为1.3 - 1.4，2至4小时后的恢复率为1.7 - 2.6。Pt(terpy)在1小时后的恢复率为1.3 - 1.6，24小时后的恢复率为1.5 - 1.8。
