Villamizar Natalia, Vera Luisa María, Foulkes Nicholas Simon, Sánchez-Vázquez Francisco Javier
1 Department of Physiology, Faculty of Biology, University of Murcia , Murcia, Spain .
Zebrafish. 2014 Apr;11(2):173-81. doi: 10.1089/zeb.2013.0926. Epub 2013 Dec 24.
In the underwater environment, the properties of light (intensity and spectrum) change rapidly with depth and water quality. In this article, we have described how and to what extent lighting conditions can influence the development, growth, and survival of zebrafish. Fertilized eggs and the corresponding larvae were exposed to different visible light wavelengths (violet, blue, green, yellow, red, and white) in a 12-h light-12-h dark (LD) cycle until 30 days posthatching (dph), when the expression of morphometric parameters and growth (igf1a, igf2a)- and stress-related (crh and pomca) genes were examined. Another group of larvae was raised under constant darkness (DD) until 5 or 10 dph, after which they were transferred to a LD of white light. A third group remained under DD to investigate the effects of light deprivation upon zebrafish development. The results revealed that the hatching rate was highest under blue and violet light, while total length at 30 dph was greatest under blue, white, and violet light. Red light led to reduced feeding activity and poor survival (100% mortality). Larvae raised under constant white light (LL) showed a higher proportion of malformations, as did larvae raised under LD violet light. The expression of growth and stress factors was upregulated in the violet (igf1a, igf2a, pomca, and chr) and blue (igf2a) groups, which is consistent with the higher growth recorded and the higher proportion of malformations detected under the violet light. All larvae kept under DD died before 18 dph, but the survival rates improved in larvae transferred to LD at 5 dph and at 10 dph. In summary, these findings revealed that lighting conditions are crucial factors influencing zebrafish larval development and growth.
在水下环境中，光的特性（强度和光谱）会随着深度和水质的变化而迅速改变。在本文中，我们描述了光照条件如何以及在多大程度上会影响斑马鱼的发育、生长和存活。受精的鱼卵及其相应的幼鱼在12小时光照-12小时黑暗（LD）循环下暴露于不同的可见光波长（紫色、蓝色、绿色、黄色、红色和白色）中，直至孵化后30天（dph），此时检测形态学参数以及生长（igf1a、igf2a）和应激相关（crh和pomca）基因的表达。另一组幼鱼在持续黑暗（DD）条件下饲养至5或10 dph，之后将它们转移到白光的LD环境中。第三组幼鱼则一直处于DD环境中，以研究光剥夺对斑马鱼发育的影响。结果显示，在蓝色和紫色光下孵化率最高，而在30 dph时，蓝色、白色和紫色光下的全长最长。红光导致摄食活动减少和存活率低（100%死亡率）。在持续白光（LL）下饲养的幼鱼以及在LD紫光下饲养的幼鱼出现畸形的比例更高。在紫色（igf1a、igf2a、pomca和chr）和蓝色（igf2a）组中，生长和应激因子的表达上调，这与在紫光下记录到的较高生长率和检测到的较高畸形比例一致。所有处于DD环境中的幼鱼在18 dph之前死亡，但在5 dph和10 dph转移到LD环境中的幼鱼存活率有所提高。总之，这些发现表明光照条件是影响斑马鱼幼鱼发育和生长的关键因素。
