伯克霍尔德氏菌属新种，从 Lebeckia ambigua 根瘤中分离得到。
Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules.
机构信息
Centre for Rhizobium Studies, Murdoch University, Murdoch, Western Australia 6150, Australia.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
出版信息
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1090-1095. doi: 10.1099/ijs.0.058602-0. Epub 2013 Dec 24.
Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556(T) being most closely related to Burkholderia caledonica LMG 23644(T) (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937(T) (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556(T) ( = LMG 27173(T) = HAMBI 3353(T)).
三株革兰氏阴性、杆状细菌从 Lebeckia ambigua 根瘤中分离出来，并在该宿主上进行了鉴定。根据 16S rRNA 基因序列系统发育树，它们属于伯克霍尔德氏菌属，代表菌株 WSM3556(T) 与 Burkholderia caledonica LMG 23644(T)（98.70% 16S rRNA 基因序列相似性）和 Burkholderia rhynchosiae WSM3937(T)（98.50%）最为密切相关。此外，这些菌株在管家基因 gyrB 和 recA 的系统发育树中形成了一个独特的群体。化学分类学数据，包括脂肪酸图谱和呼吸醌分析，支持将我们的菌株分配到伯克霍尔德氏菌属。DNA-DNA 杂交、MALDI-TOF MS 分析以及生理生化试验的结果允许我们的菌株在基因型和表型上与最接近的近缘种区分开来。因此，这些菌株代表了一个新的种，命名为 Burkholderia dilworthii sp. nov.，其模式菌株为 WSM3556(T)（=LMG 27173(T)=HAMBI 3353(T)）。
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