Centre for Invasion Biology, Department of Botany and Zoology, Natural Sciences Building, Private Bag X1, Stellenbosch University, Matieland 7602, Western Cape, South Africa.
Department of Botany and Zoology, Natural Sciences Building, Private Bag X1, Stellenbosch University, Matieland 7602, Western Cape, South Africa.
Int J Syst Evol Microbiol. 2014 Jun;64(Pt 6):1906-1912. doi: 10.1099/ijs.0.057067-0. Epub 2014 Mar 5.
During a study to investigate the diversity of rhizobia associated with native legumes in South Africa's Cape Floristic Region, a Gram-negative bacterium designated VG1C(T) was isolated from the root nodules of Aspalathus abietina Thunb. Based on phylogenetic analyses of the 16S rRNA and recA genes, VG1C(T) belongs to the genus Burkholderia, with the highest degree of sequence similarity to the type strain of Burkholderia sediminicola (98.5% and 98%, respectively). The DNA G+C content of strain VG1C(T) was 60.1 mol%, and DNA-DNA relatedness values to the type strain of closely related species were found to be substantially lower than 70%. As evidenced by results of genotypic, phenotypic and chemotaxonomic tests provided here, we conclude that isolate VG1C(T) represents a novel rhizosphere-associated species in the genus Burkholderia, for which the name Burkholderia aspalathi sp. nov. is proposed, with the type strain VG1C(T) ( = DSM 27239(T) = LMG 27731(T)).
在一项调查南非开普植物区系中与本地豆科植物共生的根瘤菌多样性的研究中，从 Aspalathus abietina Thunb 的根瘤中分离到一株革兰氏阴性细菌，命名为 VG1C(T)。根据 16S rRNA 和 recA 基因的系统发育分析，VG1C(T)属于伯克霍尔德氏菌属，与伯克霍尔德氏菌 sediminicola 的模式株的序列相似性最高（分别为 98.5%和 98%）。菌株 VG1C(T)的 DNA G+C 含量为 60.1 mol%，与亲缘关系密切的种的模式株的 DNA-DNA 同源性值明显低于 70%。根据这里提供的基因型、表型和化学分类学测试的结果，我们得出结论，分离株 VG1C(T)代表了伯克霍尔德氏菌属中一种新型的根际相关物种，建议将其命名为 Burkholderia aspalathi sp. nov.，其模式株为 VG1C(T)（=DSM 27239(T)=LMG 27731(T)）。
