Centre for Rhizobium Studies, Murdoch University, Western Australia 6150, Australia.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):3950-3957. doi: 10.1099/ijs.0.048777-0. Epub 2013 May 24.
Seven Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM5005(T) being most closely related to Burkholderia tuberum (98.08 % sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia. Results of DNA-DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest species of the genus Burkholderia with a validly published name. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (type strain WSM5005(T) = LMG 27175(T) = HAMBI 3357(T)) is proposed.
从 Lebeckia ambigua 根瘤中分离出 7 株革兰氏阴性、杆状细菌，并在该宿主上进行了鉴定。根据 16S rRNA 基因系统发育分析，它们属于伯克霍尔德氏菌属，代表菌株 WSM5005(T) 与 Burkholderia tuberum 的亲缘关系最近（序列相似度为 98.08%）。此外，这些菌株在基于管家基因 gyrB 和 recA 的系统发育树中形成了一个独特的群组。化学分类学数据包括脂肪酸图谱和呼吸醌分析支持将这些菌株归属于伯克霍尔德氏菌属。DNA-DNA 杂交、生理生化试验的结果允许我们的菌株与具有有效发表名称的伯克霍尔德氏菌属中最接近的种进行基因型和表型区分。因此，这些菌株代表了一个新的种，命名为 Burkholderia sprentiae sp. nov.（模式菌株 WSM5005(T) = LMG 27175(T) = HAMBI 3357(T)）。
