Centre for Rhizobium Studies, Murdoch University, Western Australia 6150, Australia.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):3944-3949. doi: 10.1099/ijs.0.048751-0. Epub 2013 May 24.
Two strains of Gram-stain-negative, rod-shaped bacteria were isolated from root nodules of the South African legume Rhynchosia ferulifolia and authenticated on this host. Based on phylogenetic analysis of the 16S rRNA gene, strains WSM3930 and WSM3937(T) belonged to the genus Burkholderia, with the highest degree of sequence similarity to Burkholderia terricola (98.84 %). Additionally, the housekeeping genes gyrB and recA were analysed since 16S rRNA gene sequences are highly similar between closely related species of the genus Burkholderia. The results obtained for both housekeeping genes, gyrB and recA, showed the highest degree of sequence similarity of the novel strains towards Burkholderia caledonica LMG 19076(T) (94.2 % and 94.5 %, respectively). Chemotaxonomic data, including fatty acid profiles and respiratory quinone data supported the assignment of strains WSM3930 and WSM3937(T) to the genus Burkholderia. DNA-DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains WSM3930 and WSM3937(T) from the most closely related species of the genus Burkholderia with validly published names. We conclude, therefore, that these strains represent a novel species for which the name Burkholderia rhynchosiae sp. nov. is proposed, with strain WSM3937(T) ( = LMG 27174(T) = HAMBI 3354(T)) as the type strain.
两株革兰氏阴性、杆状细菌从南非豆科植物 Rhynchosia ferulifolia 的根瘤中分离出来，并在该宿主上进行了鉴定。基于 16S rRNA 基因的系统发育分析，菌株 WSM3930 和 WSM3937(T) 属于伯克霍尔德氏菌属，与伯克霍尔德氏菌 terricola 的序列相似性最高（98.84%）。此外，还分析了管家基因 gyrB 和 recA，因为伯克霍尔德氏菌属的密切相关物种之间的 16S rRNA 基因序列高度相似。两个管家基因 gyrB 和 recA 的结果表明，新菌株与 Burkholderia caledonica LMG 19076(T) 的序列相似性最高（分别为 94.2%和 94.5%）。化学分类学数据，包括脂肪酸图谱和呼吸醌数据，支持将菌株 WSM3930 和 WSM3937(T) 分配到伯克霍尔德氏菌属。DNA-DNA 杂交以及生理生化试验允许菌株 WSM3930 和 WSM3937(T) 在种水平上与最密切相关的伯克霍尔德氏菌属物种进行基因和表型分化，这些物种具有有效发表的名称。因此，我们得出结论，这些菌株代表一个新的种，建议采用 Burkholderia rhynchosiae sp. nov. 这个名称，其中菌株 WSM3937(T)（=LMG 27174(T)=HAMBI 3354(T)）作为模式菌株。
