Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P
Exp Cell Res. 1987 May;170(1):235-49. doi: 10.1016/0014-4827(87)90133-9.
Monoclonal antibody (RCK 105) directed against keratin 7 was obtained after immunization of BALB/c mice with cytoskeletal preparations from T24 cells and characterized by one- (1D) and two-dimensional (2D) immunoblotting. In cultured epithelial cells, known from gel electrophoretic studies to contain keratin 7, this antibody gives a typical keratin intermediate filament staining pattern, comparable to that obtained with polyclonal rabbit antisera to skin keratins or with other monoclonal antibodies, recognizing for example keratins 5 and 8 or keratin 18. Using RCK 105, the distribution of keratin 7 throughout human epithelial tissues was examined and correlated with expression patterns of other keratins. Keratin 7 was found to occur in the columnar and glandular epithelium of the lung, cervix, breast, in bile ducts, collecting ducts in the kidney and in mesothelium, but to be absent from gastrointestinal epithelium, hepatocytes, proximal and distal tubules of the kidney and myoepithelium. Nor could it be detected in the stratified epithelia of the skin, tongue, esophagus, or cervix but strongly stained all cell layers of the urinary bladder transitional epithelium. When applied to carcinomas derived from these different tissue types it became obvious that an antibody to keratin 7 may allow an immunohistochemical distinction between certain types of adenocarcinomas.
用T24细胞的细胞骨架制剂免疫BALB/c小鼠后，获得了针对角蛋白7的单克隆抗体（RCK 105），并通过一维（1D）和二维（2D）免疫印迹进行了表征。在凝胶电泳研究中已知含有角蛋白7的培养上皮细胞中，该抗体呈现出典型的角蛋白中间丝染色模式，与用针对皮肤角蛋白的多克隆兔抗血清或其他单克隆抗体（例如识别角蛋白5和8或角蛋白18的抗体）所获得的模式相当。使用RCK 105，研究了角蛋白7在整个人体上皮组织中的分布，并与其他角蛋白的表达模式进行了关联。发现角蛋白7存在于肺、子宫颈、乳腺的柱状和腺上皮、胆管、肾集合管以及间皮中，但在胃肠道上皮、肝细胞、肾近端和远端小管以及肌上皮中不存在。在皮肤、舌头、食道或子宫颈的复层上皮中也检测不到，但膀胱移行上皮的所有细胞层均被强烈染色。当应用于源自这些不同组织类型的癌时，很明显，角蛋白7抗体可用于某些类型腺癌的免疫组织化学鉴别。
