de las Mulas J, Espinosa de los Monteros A, Gomez-Villamandos J C, Fernandez A, Vos J H
Department of Comparative Pathology, Veterinary Faculty, University of Córdoba.
Zentralbl Veterinarmed A. 1994 May;41(4):283-97. doi: 10.1111/j.1439-0442.1994.tb00095.x.
The immunohistochemical distribution pattern of some keratin intermediate filament proteins has been analysed in a wide range of formalin fixed, paraffin embedded feline tissues using one polyclonal and two monoclonal antibodies raised against human keratins by means of the avidin-biotin-peroxidase complex technique. Only the epithelial and mesothelial cells were stained by the three antibodies, but differences in their corresponding staining pattern were noticed. The staining reaction of the polyclonal antibody raised against human skin keratin was found in both stratified and complex epithelia, while that of the monoclonal antibody which recognizes human keratins 8 + 18 + 19 of the Moll catalogue (NCL-5D3) was restricted to some simple epithelia. The staining reaction of the monoclonal antibody which reacts with human keratins 5 + 8 of the Moll catalogue (RCK-102) covered the widest spectrum of feline epithelial tissues analysed, including stratified, complex and simple epithelia. These staining patterns of feline tissues are basically similar with respect to those of corresponding tissues in other mammalian species, although some differences were also noticed and some obvious epithelial tissues were not stained. This study confirms the broad interspecies cross-reactivity of keratin proteins antibodies and demonstrates their capability to differentiate between various types of feline epithelia and some epithelial compartments.
利用抗人角蛋白产生的一种多克隆抗体和两种单克隆抗体，通过抗生物素蛋白-生物素-过氧化物酶复合物技术，对大量福尔马林固定、石蜡包埋的猫科动物组织中某些角蛋白中间丝蛋白的免疫组织化学分布模式进行了分析。三种抗体仅对上皮细胞和间皮细胞进行了染色，但它们相应的染色模式存在差异。发现针对人皮肤角蛋白产生的多克隆抗体的染色反应出现在复层上皮和复层上皮中，而识别莫尔目录（NCL-5D3）中人角蛋白8 + 18 + 19的单克隆抗体的染色反应仅限于一些单层上皮。与莫尔目录（RCK-102）中人角蛋白5 + 8反应的单克隆抗体的染色反应覆盖了所分析的猫科动物上皮组织的最广泛范围，包括复层上皮、复层上皮和单层上皮。尽管也注意到了一些差异，并且一些明显的上皮组织未被染色，但猫科动物组织的这些染色模式与其他哺乳动物物种相应组织的染色模式基本相似。这项研究证实了角蛋白抗体具有广泛的种间交叉反应性，并证明了它们区分不同类型猫科动物上皮和一些上皮隔室的能力。
