Noumoff J, Haydock S W, Sachdeva R, Heyner S, Pritchard M L
Gynecol Oncol. 1987 Jun;27(2):141-9. doi: 10.1016/0090-8258(87)90286-1.
Five established cell lines of human endometrium, two of normal endometrium and three of proven tumorigenicity, have been compared in terms of morphology and chromosomal numbers. Each of the five cell lines was then analyzed using immunocytochemical techniques to show that the epithelial and stromal elements could be separately identified. Antibodies directed against cytokeratin and desmoplakins were used to identify epithelial elements and antibodies directed against fibronectin were used as a marker for stroma. These results were then confirmed using Western blot analysis. We conclude that cell lines of human endometrium in culture can be differentiated as being of epithelial or stromal origin. Cell lines derived from reportedly normal human endometrium exhibit a stromal phenotype with a normal karyotype, whereas cells of tumorigenic human endometrial cell lines exhibit an epithelial phenotype and abnormal karyologic characteristics.
已对五种已建立的人子宫内膜细胞系进行了形态学和染色体数目方面的比较，其中两种为正常子宫内膜细胞系，三种具有致瘤性。然后使用免疫细胞化学技术对这五种细胞系中的每一种进行分析，以表明上皮和基质成分可以被分别识别。针对细胞角蛋白和桥粒斑蛋白的抗体用于识别上皮成分，针对纤连蛋白的抗体用作基质的标志物。然后使用蛋白质免疫印迹分析对这些结果进行了确认。我们得出结论，培养中的人子宫内膜细胞系可以根据其上皮或基质来源进行区分。源自据报道正常的人子宫内膜的细胞系表现出具有正常核型的基质表型，而致瘤性人子宫内膜细胞系的细胞表现出上皮表型和异常的核学特征。
