Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
Anal Chim Acta. 2014 Jan 15;808:144-50. doi: 10.1016/j.aca.2013.09.037. Epub 2013 Oct 4.
Polyamidoamine (PAMAM) are synthetic dendrimers which present attractive properties for the biological and biomedical fields, as they proved to be efficient drug and gene carriers. In order to increase their transfection efficiency, chemical modifications of the amino end-groups had been reported. In this work, the synthesis of the ammonia-cored G1(N) PAMAM and the consecutive chemical modification with glycine or phenylalanine amino-acids were monitored using the coupling of thin layer chromatography (TLC) with matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). Thus, the monitoring of the PAMAM synthesis included the identification of the by-products such as defective structures of PAMAM dendrimers as well as the study of phenylalanine-grafted PAMAM oligomer distribution.
聚酰胺-胺（PAMAM）是一种合成的树状大分子，具有在生物和生物医学领域的吸引力，因为它们被证明是有效的药物和基因载体。为了提高其转染效率，已经报道了对氨基末端基团进行化学修饰。在这项工作中，使用薄层层析（TLC）与基质辅助激光解吸电离-质谱（MALDI-MS）相结合，监测了氨核 G1（N）PAMAM 的合成以及随后与甘氨酸或苯丙氨酸氨基酸的化学修饰。因此，PAMAM 合成的监测包括鉴定副产物，如 PAMAM 树状大分子的缺陷结构，以及研究苯丙氨酸接枝 PAMAM 低聚物的分布。
