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使用多种基质组合提高基质辅助激光解吸电离飞行时间质谱法中树枝状大分子鉴定的灵敏度和分辨率

Improving Sensitivity and Resolution of Dendrimer Identification in MALDI-TOF Mass Spectrometry Using Varied Matrix Combinations.

作者信息

Sanhueza Claudia, Dias Nathalia Baptista, Vergara Daniela, Silva Lisette, Chávez-Ángel Emigdio, Castro-Alvarez Alejandro

机构信息

Center for Resilience, Adaptation and Mitigation (CReAM), Faculty of Sciences, Universidad Mayor, Temuco 4780000, Chile.

Escuela de Ingeniería, Facultad de Ciencias, Ingeniería y Tecnología, Universidad Mayor, Temuco 4780000, Chile.

出版信息

Polymers (Basel). 2025 Jan 16;17(2):219. doi: 10.3390/polym17020219.

DOI:10.3390/polym17020219
PMID:39861291
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11769133/
Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a well-known technique for polymer analysis, particularly for determining the molecular weight and structural details of dendrimers. In this study, we evaluated the performance of various matrices, such as 2',4',6'-trihydroxyacetophenone (THAP), α-cyano-4-hydroxycinnamic acid (HCCA), and sinapinic acid (SA), and their combinations, on the sensitivity and resolution of poly(amidoamine) (PAMAM) dendrimers of different generations (G3.0, G4.0, and G5.0). Our results demonstrated that the combination of HCCA-THAP significantly enhanced spectral resolution and peak intensity compared to individual matrices, particularly for higher-generation dendrimers. This improvement is attributed to the better ionization efficiency achieved with the combined matrices. These findings provide critical insights into optimizing MALDI-TOF MS for the accurate characterization of complex polymers, with potential applications in drug delivery and nanotechnology.

摘要

基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）是一种用于聚合物分析的知名技术，尤其适用于测定树枝状大分子的分子量和结构细节。在本研究中，我们评估了各种基质，如2',4',6'-三羟基苯乙酮（THAP）、α-氰基-4-羟基肉桂酸（HCCA）和芥子酸（SA）及其组合，对不同代数（G3.0、G4.0和G5.0）的聚（酰胺胺）（PAMAM）树枝状大分子的灵敏度和分辨率的影响。我们的结果表明，与单个基质相比，HCCA-THAP的组合显著提高了光谱分辨率和峰强度，特别是对于更高代数的树枝状大分子。这种改进归因于组合基质实现了更好的电离效率。这些发现为优化MALDI-TOF MS以准确表征复杂聚合物提供了关键见解，在药物递送和纳米技术中具有潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/78d8fad579ad/polymers-17-00219-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/c08733efe03f/polymers-17-00219-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/fb5872f4d632/polymers-17-00219-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/33791b174a25/polymers-17-00219-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/c78d2c566af4/polymers-17-00219-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/fa830ede5a6f/polymers-17-00219-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/78d8fad579ad/polymers-17-00219-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/c08733efe03f/polymers-17-00219-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/fb5872f4d632/polymers-17-00219-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/33791b174a25/polymers-17-00219-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/c78d2c566af4/polymers-17-00219-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/fa830ede5a6f/polymers-17-00219-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a99/11769133/78d8fad579ad/polymers-17-00219-g006.jpg

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本文引用的文献

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and studies of gp120-HIV-derived peptides in complex with G4-PAMAM dendrimers.以及对与G4-PAMAM树枝状大分子复合的gp120-HIV衍生肽的研究。
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PAMAM Dendrimer Nanomolecules Utilized as Drug Delivery Systems for Potential Treatment of Glioblastoma: A Systematic Review.PAMAM 树枝状纳米分子作为递药系统用于胶质母细胞瘤的潜在治疗：系统评价。
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