Wu De-sheng, Yang Lin-qing, Huang Sui, Liu Jian-jun, Xu Xin-yun, Huang Hai-yan, Gong Chun-mei, Hu Gong-hua, Liu Qing-cheng, Yang Xi-fei, Hong Wen-xu, Zhou Li, Huang Xin-feng, Yuan Jian-hui, Zhuang Zhi-xiong
Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China. E-mail:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Nov;31(11):811-4.
To study in vitro sperm damage caused by trichloroethylene in male rats.
Sperms of Sprague-Dawley (SD) rats were collected 4 hours after being contaminated by trichloroethylene of 0, 2, 4, 6, 8, and 10 mmol/L in vitro. Giemsa staining was performed to observe the morphological changes of sperms, and flow cytometer was used to detect the changes in mitochondrial membrane potential.
The sperm motilities in 6, 8, and 10 mmol/L trichloroethylene groups decreased significantly compared with that in control group (P <0.01); the sperm aberration rates in 8 and 10 mmol/L trichloroethylene groups were significantly higher than that in control group (P<0.01). With the increase in exposure dose, the proportion of sperms with reduced mitochondrial membrane potential increased, and there were significant differences in sperm apoptosis rate between the 4, 6, 8, and 10 mmol/L trichloroethylene groups and control group (P<0.01).
In vitro exposure to trichloroethylene can reduce sperm motility and increase the aberration rate and apoptosis rate of sperms in male SD rats.
研究三氯乙烯对雄性大鼠精子的体外损伤作用。
将体外培养的Sprague-Dawley（SD）大鼠精子分别用0、2、4、6、8和10 mmol/L的三氯乙烯污染4小时后，进行吉姆萨染色观察精子形态变化，并用流式细胞仪检测线粒体膜电位的变化。
6、8和10 mmol/L三氯乙烯组精子活力较对照组显著降低（P<0.01）；8和10 mmol/L三氯乙烯组精子畸变率显著高于对照组（P<0.01）。随着染毒剂量增加，线粒体膜电位降低的精子比例增加，4、6、8和10 mmol/L三氯乙烯组与对照组精子凋亡率比较差异有统计学意义（P<0.01）。
体外染毒三氯乙烯可降低雄性SD大鼠精子活力，增加精子畸变率和凋亡率。
