Agnihotri Saurabh Kumar, Agrawal Ankit Kumar, Hakim Bilal Ahmad, Vishwakarma A L, Narender T, Sachan Rekha, Sachdev Monika
Endocrinology Division, CSIR- Central Drug Research Institute, Lucknow, UP, 226 031, India.
Sophisticated Analytical Instrument Facility, CSIR- Central Drug Research Institute, Lucknow, UP, India.
In Vitro Cell Dev Biol Anim. 2016 Oct;52(9):953-960. doi: 10.1007/s11626-016-0061-x. Epub 2016 Jun 23.
Sperm motility is the major decisive factor in determining male fertility. The objective of the present study was to analyse the effect of mitochondrial membrane potential (MMP) on the temporal regulation of sperm motility. Observations were recorded in various rodent species and among differentially motile sperm fractions including swim up and leftover layer of human semen sample using JC-1 stain (a marker of the MMP) through FACS. Swim-up sperms having highest motility showed significantly higher MMP as compared to leftover sperms, which had the least motility. Interestingly, infertile patients with compromised motility showed low MMP as compared to the healthy individuals. Further, as per the time lapse, sperm motility goes down, at the same time, it was observed that MMP also decreases in human as well as in rodent sperms. Treatment of known spermicides on human sperms reduced their motility drastically which in turn also reduced its MMP significantly. Treatment of human sperms with oxidative uncoupler also impeded their motility by reducing MMP, indicating a definitive role on MMP on sperm motility and fertility. Based on the results of the study, MMP can be considered as a potential regulator and indicator of sperm motility and hence could be directly related to male fertility.
精子活力是决定男性生育能力的主要决定性因素。本研究的目的是分析线粒体膜电位（MMP）对精子活力时间调控的影响。使用JC-1染色剂（一种MMP的标志物）通过流式细胞术（FACS）在各种啮齿动物物种以及不同活力的精子组分中进行观察，这些组分包括人精液样本的上浮层和剩余层。与活力最低的剩余精子相比，活力最高的上浮精子显示出显著更高的MMP。有趣的是，与健康个体相比，活力受损的不育患者显示出较低的MMP。此外，随着时间的推移，精子活力下降，与此同时，观察到人类和啮齿动物精子中的MMP也会降低。用已知的杀精剂处理人类精子会大幅降低其活力，这反过来也会显著降低其MMP。用氧化解偶联剂处理人类精子也会通过降低MMP来阻碍其活力，表明MMP对精子活力和生育能力具有决定性作用。基于该研究结果，MMP可被视为精子活力的潜在调节因子和指标，因此可能与男性生育能力直接相关。
