Miao Rong-ming, Ding Bang-mei, Zhang Xue-tao, Fang Zhong-hua, Zhao Rui, Zhang Ying-yi, Zhao Dao-kun
Wuxi No.8 People's Hospital (Wuxi Hospital for Prevention and Treatment of Occupational Disease), 2140028, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Dec;31(12):924-6.
To observe the changes in activities of superoxide dismutase (SOD) and glutathione peroxide (GSH-Px) in the induced sputum of silicosis patients, and to investigate the roles of SOD and GSH-Px in the development and progression of silicosis and the significance of measuring activities of SOD and GSH-Px in induced sputum among silicosis patients.
Fifty hotel attendants were chosen as control group, 50 workers with more than one year of silica dust exposure as dust exposure group, 32 silica dust-exposed workers as observation subject group, and 52 silicosis patients as silicosis group. The activities of SOD and GSH-Px in their induced sputum were measured by enzyme-linked immunosorbent assay.
Compared with the control group, the observation subject group and silicosis group had significantly decreased SOD activity (68.16 ± 30.17 and 66.38 ± 47.32 U/ml vs 75.81 ± 11.92 U/ml, P < 0.05); compared with the dust exposure group, the silicosis group had significantly decreased SOD activity (66.38 ± 47.32 U/ml vs 70.12 ± 14.31 U/ml, P < 0.05). Compared with the control group and dust exposure group, the observation subject group and silicosis group had significantly increased GSH-Px activity (268.21 ± 15.45 and 279.34 ± 29.26 U/ml vs 224.22 ± 12.64 and 236.41 ± 14.54 U/ml, P < 0.05 or P < 0.01).
The SOD activity in dust exposure group and silicosis group decreased, but there were no significant differences between patients with different stages of silicosis. The GSH-Px activity in dust exposure group and silicosis group was significantly higher than that in control group, and there were significant differences between patients with different stages of silicosis. These suggest that the imbalance of oxidative/antioxidant systems is associated with the development and progression of silicosis.
观察矽肺患者诱导痰中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性的变化，探讨SOD和GSH-Px在矽肺发生发展中的作用以及检测矽肺患者诱导痰中SOD和GSH-Px活性的意义。
选取50名酒店服务员作为对照组，50名有1年以上二氧化硅粉尘接触史的工人作为粉尘接触组，32名二氧化硅粉尘接触工人作为观察对象组，52名矽肺患者作为矽肺组。采用酶联免疫吸附测定法检测其诱导痰中SOD和GSH-Px的活性。
与对照组相比，观察对象组和矽肺组SOD活性显著降低（68.16±30.17和66.38±47.32 U/ml vs 75.81±11.92 U/ml，P<0.05）；与粉尘接触组相比，矽肺组SOD活性显著降低（66.38±47.32 U/ml vs 70.12±14.31 U/ml，P<0.05）。与对照组和粉尘接触组相比，观察对象组和矽肺组GSH-Px活性显著升高（268.21±15.45和279.34±29.26 U/ml vs 224.22±12.64和236.41±14.54 U/ml，P<0.05或P<0.01）。
粉尘接触组和矽肺组SOD活性降低，但不同期矽肺患者之间差异无统计学意义。粉尘接触组和矽肺组GSH-Px活性显著高于对照组，且不同期矽肺患者之间差异有统计学意义。这表明氧化/抗氧化系统失衡与矽肺的发生发展有关。
