Stadler B M, Hirai K, Brantschen S, Nakajima K, de Weck A L
Int Arch Allergy Appl Immunol. 1987;82(3-4):338-40. doi: 10.1159/000234220.
Human T cells produce a factor that induces growth of metachromatically staining cells in human bone marrow cultures. These cultured cells contain metachromatically staining granules and release histamine upon triggering with IgE and anti-IgE antibody. Based on morphological criteria, these cultured cells were termed basophil-like cells. We have generated a human T hybridoma which produces this basophil-like cell-promoting activity (BaPA). BaPA has a molecular weight of approximately 20 kilodaltons and isoelectric points between pH 5.8 and 7.5, with a major peak at pH 7. BaPA is of protein nature and can be clearly separated from interleukin-1 (IL-1), IL-2, interferons, GM-CSF and M-CSF. BaPA is also clearly different from a human IL-3-like activity which by itself can induce growth of metachromatically staining cells containing lower histamine levels than the cells cultured in the presence of BaPA. The growth of human basophil/mast cell-like cells can furthermore be enhanced if human bone marrow cells are cultured in the presence of fibroblast feeder cells.
人T细胞产生一种因子，可诱导人骨髓培养物中异染性染色细胞的生长。这些培养细胞含有异染性染色颗粒，并在受到IgE和抗IgE抗体触发时释放组胺。根据形态学标准，这些培养细胞被称为嗜碱性粒细胞样细胞。我们已经产生了一种产生这种嗜碱性粒细胞样细胞促进活性（BaPA）的人T杂交瘤。BaPA的分子量约为20千道尔顿，等电点在pH 5.8至7.5之间，在pH 7处有一个主峰。BaPA具有蛋白质性质，并且可以与白细胞介素-1（IL-1）、IL-2、干扰素、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和巨噬细胞集落刺激因子（M-CSF）明显分离。BaPA也明显不同于一种人IL-3样活性，后者本身可诱导异染性染色细胞的生长，这些细胞所含组胺水平低于在BaPA存在下培养的细胞。此外，如果人骨髓细胞在成纤维细胞饲养细胞存在下培养，人嗜碱性粒细胞/肥大细胞样细胞的生长可以得到增强。
