Stadler B M, Hirai K, Tadokoro K, de Weck A L
Int Arch Allergy Appl Immunol. 1985;77(1-2):151-4. doi: 10.1159/000233770.
Lectin-stimulated supernatants of human peripheral blood leukocytes (PBL) contained an activity that maintained the long-term growth of murine interleukin-3 (IL-3) dependent cell lines such as the 32 Dcl line. We compared this human IL-3 activity with the basophil promoting activity (BaPA) recently described by us. Besides lectin-stimulated PBL supernatants that contained both BaPa and IL-3, we found that BaPA was produced mainly by E rosette-positive T cells or the human Mo T cell line, while IL-3 was produced by human T hybridomas and, more interestingly, by human macrophage cell lines. Based on molecular weight (BaPA: 20-30 kilodaltons, IL-3: 15-19 kilodaltons) as well as on molecular charge (BaPA: pI = 6.1-7.5, IL-3: 4.9-6.2), we concluded that human IL-3 and BaPA are distinct factors.
人外周血白细胞（PBL）经凝集素刺激后的上清液中含有一种活性物质，该物质可维持小鼠白细胞介素-3（IL-3）依赖细胞系（如32 Dcl细胞系）的长期生长。我们将这种人IL-3活性与我们最近描述的嗜碱性粒细胞促进活性（BaPA）进行了比较。除了含有BaPa和IL-3的凝集素刺激的PBL上清液外，我们发现BaPA主要由E玫瑰花结阳性T细胞或人Mo T细胞系产生，而IL-3则由人T杂交瘤产生，更有趣的是，还由人巨噬细胞系产生。基于分子量（BaPA：20 - 30千道尔顿，IL-3：15 - 19千道尔顿）以及分子电荷（BaPA：等电点 = 6.1 - 7.5，IL-3：4.9 - 6.2），我们得出结论，人IL-3和BaPA是不同的因子。
