Pearce F L, Flint K C, Leung K B, Hudspith B N, Seager K, Hammond M D, Brostoff J, Geraint-James D, Johnson N M
Int Arch Allergy Appl Immunol. 1987;82(3-4):507-12. doi: 10.1159/000234266.
Human pulmonary mast cells were obtained by bronchoalveolar lavage (BAL) and by enzymic dissociation of whole lung. The cells released histamine on immunological stimulation or on exposure to a hyperosmolar environment. Cell suspensions similarly released newly generated products of arachidonic acid metabolism. Increased numbers of mast cells were recovered by BAL of asthmatic subjects and patients suffering from sarcoidosis and these cells were hyperresponsive to immunological challenge. Mast cells recovered by BAL and enzymic dissociation were differentially inhibited by antiasthmatic drugs. These data emphasize the potential role of BAL mast cells in pulmonary diseases of diverse origin.
通过支气管肺泡灌洗（BAL）和全肺酶解获得人肺肥大细胞。这些细胞在免疫刺激或暴露于高渗环境时会释放组胺。细胞悬液同样会释放新生成的花生四烯酸代谢产物。通过对哮喘患者和结节病患者进行BAL可回收更多数量的肥大细胞，并且这些细胞对免疫攻击反应过度。通过BAL和酶解回收的肥大细胞受到抗哮喘药物的不同抑制。这些数据强调了BAL肥大细胞在多种起源的肺部疾病中的潜在作用。
