Hood A T, Currie D, Garte S J
In Vitro Cell Dev Biol. 1987 Apr;23(4):274-8. doi: 10.1007/BF02623710.
A new cell line designated NAS 2BL has been established from a rat nasal tumor induced by inhalation of the direct-acting carcinogen methylmethane sulfonate. The cells are epithelial in morphology, have a generation time of 34 h, require 10% fetal bovine serum for optimal growth, and exhibit keratinization at confluence. The karyotype is aneuploid, with several marker chromosomes, and the cells are transformed by the criterion of nude mouse tumorigenicity.
一种名为NAS 2BL的新细胞系是从吸入直接作用致癌物甲磺酸甲酯诱导的大鼠鼻肿瘤中建立的。这些细胞在形态上为上皮细胞，倍增时间为34小时，最佳生长需要10%胎牛血清，汇合时表现出角质化。核型为非整倍体，有几条标记染色体，根据裸鼠致瘤性标准，这些细胞已发生转化。
