Steele V E, Arnold J T
In Vitro Cell Dev Biol. 1985 Dec;21(12):681-7. doi: 10.1007/BF02620922.
Nasal turbinate epithelial cells were isolated from rats, rabbits, and humans using either a surgical or an in situ enzyme incubation technique. The culture conditions that permit optimal cell attachment and selective growth of the nasal epithelial cells were determined. These conditions will permit the long-term culture of these cells where typically 20 to 30 population doublings were observed. Differences between rat and human nasal epithelial cells were seen in substrate requirements, colony-forming efficiency, and response to fetal bovine serum and bovine serum albumin. These methodology and results will permit mechanistic studies of normal and abnormal cellular function and comparative response studies between nasal epithelial cells from rats and humans.
使用手术或原位酶孵育技术从大鼠、兔子和人类中分离出鼻甲上皮细胞。确定了允许鼻上皮细胞最佳附着和选择性生长的培养条件。这些条件将允许对这些细胞进行长期培养，在此期间通常可观察到20至30次群体倍增。在底物需求、集落形成效率以及对胎牛血清和牛血清白蛋白的反应方面，观察到大鼠和人类鼻上皮细胞之间存在差异。这些方法和结果将有助于对正常和异常细胞功能进行机制研究，以及对大鼠和人类鼻上皮细胞之间的比较反应研究。
