Yu Dianyu, Ma Ying, Jiang Lianzhou, Walid Elfalleh, He Shenghua, He Yanming, Xiaoyu Zhou, Zhang Jianing, Hu Lizhi
School of Food Science and Engineering, Harbin Institute of Technology.
J Oleo Sci. 2014;63(1):25-30. doi: 10.5650/jos.ess13105. Epub 2013 Dec 24.
The aim of this study was evaluation of stability of immobilized phospholipase A2 (PLA2) for soybean oil degumming. Also, the effect of reaction time on residual phosphorus levels was investigated according to the optimum pH and temperature. The free PLA2 and three immobilized PLA2 demonstrated significant differences in optimum operation conditions. pH, temperature and reaction time increased upon immobilization for three different immobilized PLA2 (PLA2-CA, PLA2-CAC and PLA2-CAG). Immobilized PLA2 showed enhanced thermal stability and retained more than 74% of relative activity after 1 h of incubation at 60°C, while the free PLA2 retained only 33%. The three immobilized PLA2 retained 30% to 60% of initial activities after 7 recycles. In particular, PLA2-CAC has more significant profiles in pH, temperature, reaction time and showed the highest remaining activity, thermal stability, reusability. Therefore, PLA2-CAC is a suitable immobilized enzyme for soybean oil degumming process.
本研究的目的是评估固定化磷脂酶A2（PLA2）用于大豆油脱胶的稳定性。此外，根据最佳pH值和温度，研究了反应时间对残留磷含量的影响。游离PLA2和三种固定化PLA2在最佳操作条件上表现出显著差异。对于三种不同的固定化PLA2（PLA2-CA、PLA2-CAC和PLA2-CAG），固定化后pH值、温度和反应时间均有所增加。固定化PLA2表现出增强的热稳定性，在60°C孵育1小时后保留了超过74%的相对活性，而游离PLA2仅保留了33%。三种固定化PLA2在7次循环后保留了30%至60%的初始活性。特别是，PLA2-CAC在pH值、温度、反应时间方面具有更显著的特征，并且表现出最高的剩余活性、热稳定性和可重复使用性。因此，PLA2-CAC是大豆油脱胶过程中一种合适的固定化酶。
