Waller Jessica L, Diaz Maureen H, Petrone Brianna L, Benitez Alvaro J, Wolff Bernard J, Edison Laura, Tobin-D'Angelo Melissa, Moore Ashley, Martyn Audrey, Dishman Hope, Drenzek Cherie L, Turner Kim, Hicks Lauri A, Winchell Jonas M
Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
J Clin Microbiol. 2014 Mar;52(3):849-53. doi: 10.1128/JCM.02810-13. Epub 2013 Dec 26.
An outbreak at a university in Georgia was identified after 83 cases of probable pneumonia were reported among students. Respiratory specimens were obtained from 21 students for the outbreak investigation. The TaqMan array card (TAC), a quantitative PCR (qPCR)-based multipathogen detection technology, was used to initially identify Mycoplasma pneumoniae as the causative agent in this outbreak. TAC demonstrated 100% diagnostic specificity and sensitivity compared to those of the multiplex qPCR assay for this agent. All M. pneumoniae specimens (n=12) and isolates (n=10) were found through genetic analysis to be susceptible to macrolide antibiotics. The strain diversity of M. pneumoniae associated with this outbreak setting was identified using a variety of molecular typing procedures, resulting in two P1 genotypes (types 1 [60%] and 2 [40%]) and seven different multilocus variable-number tandem-repeat analysis (MLVA) profiles. Continued molecular typing of this organism, particularly during outbreaks, may enhance the current understanding of the epidemiology of M. pneumoniae and may ultimately lead to a more effective public health response.
佐治亚州一所大学报告了83例疑似肺炎病例后，确认发生了疫情。从21名学生身上采集了呼吸道标本用于疫情调查。TaqMan阵列卡（TAC）是一种基于定量PCR（qPCR）的多病原体检测技术，最初用于确定此次疫情的病原体为肺炎支原体。与该病原体的多重qPCR检测相比，TAC显示出100%的诊断特异性和敏感性。通过基因分析发现，所有肺炎支原体标本（n = 12）和分离株（n = 10）对大环内酯类抗生素敏感。使用多种分子分型方法确定了与此次疫情相关的肺炎支原体菌株多样性，结果显示有两种P1基因型（1型[60%]和2型[40%]）以及七种不同的多位点可变数目串联重复分析（MLVA）谱型。对该病原体持续进行分子分型，尤其是在疫情期间，可能会加深目前对肺炎支原体流行病学的了解，并最终可能带来更有效的公共卫生应对措施。
