Premasiri W R, Sauer-Budge A F, Lee J C, Klapperich C M, Ziegler L D
Department of Chemistry, 590 Commonwealth Ave., Boston University, Boston MA 02215 ; Photonics Center, 15 Saint Mary's St., Boston University, Boston MA 02215.
Photonics Center, 15 Saint Mary's St., Boston University, Boston MA 02215 ; Fraunhofer USA - Center for Manufacturing Innovation, 15 Saint Mary's St. Brookline, MA 02446 ; Department of Biomedical Engineering, 44 Cummington St., Boston University, Boston MA 02215.
Spectroscopy (Springf). 2012 Jun 1;27(6):s8-31.
There is a continuing need to develop new techniques for the rapid and specific identification of bacterial pathogens in human body fluids especially given the increasing prevalence of drug resistant strains. Efforts to develop a surface enhanced Raman spectroscopy (SERS) based approach, which encompasses sample preparation, SERS substrates, portable Raman microscopy instrumentation and novel identification software, are described. The progress made in each of these areas in our laboratory is summarized and illustrated by a spiked infectious sample for urinary tract infection (UTI) diagnostics. SERS bacterial spectra exhibit both enhanced sensitivity and specificity allowing the development of an easy to use, portable, optical platform for pathogen detection and identification. SERS of bacterial cells is shown to offer not only reproducible molecular spectroscopic signatures for analytical applications in clinical diagnostics, but also is a new tool for studying biochemical activity in real time at the outer layers of these organisms.
持续需要开发新技术，以便快速、特异性地鉴定人体体液中的细菌病原体，尤其是考虑到耐药菌株的日益流行。本文描述了基于表面增强拉曼光谱（SERS）的方法的研发工作，该方法涵盖样品制备、SERS基底、便携式拉曼显微镜仪器和新型识别软件。通过用于尿路感染（UTI）诊断的加标感染样品，总结并说明了我们实验室在这些领域中取得的进展。SERS细菌光谱显示出更高的灵敏度和特异性，从而能够开发出一种易于使用、便携式的光学平台，用于病原体检测和鉴定。细菌细胞的SERS不仅为临床诊断中的分析应用提供了可重复的分子光谱特征，而且还是实时研究这些生物体外层生化活性的新工具。
