Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.
Mediators Inflamm. 2013;2013:928137. doi: 10.1155/2013/928137. Epub 2013 Nov 24.
The aim of this study was to determine the effect of azithromycin on LPS-induced pregnancy loss. Thirty-six pregnant female Wistar rats were divided into 4 equal groups as follows: control group, where 0.3 mL of normal saline solution was administered intravenously on day 10 of pregnancy; azithromycin group, where azithromycin was administered orally at 350 mg kg(-1) day on days 9, 10, and 11 of pregnancy; lipopolysaccharide group, where LPS was administered intravenously via the tail vein at 160 μg kg(-1) on day 10 of pregnancy; and the azithromycin + LPS group, where azithromycin was administered orally at 350 mg kg(-1) day on days 9, 10, and 11 of pregnancy and LPS was administered intravenously at 160 μg kg(-1) on day 10 of pregnancy. Blood samples were obtained from the tail vein on day 10 of the experiment. Pregnancy rates were determined. Tumor necrosis factor-alpha (TNF- α ) and interleukin (IL-10) levels were measured by ELISA. Azithromycin prevented (P < 0.05) LPS-induced pregnancy loss. Higher TNF- α and IL-10 levels were measured (P < 0.05) in the LPS and azithromycin + LPS groups, respectively. In conclusion, azithromycin may be useful in infection- or endotoxemia-dependent pregnancy loss.
本研究旨在探讨阿奇霉素对脂多糖诱导的妊娠丢失的影响。将 36 只妊娠雌性 Wistar 大鼠随机分为 4 组，每组 9 只：对照组，妊娠第 10 天静脉注射 0.3ml 生理盐水；阿奇霉素组，妊娠第 9、10、11 天口服阿奇霉素 350mg/kg；脂多糖组，妊娠第 10 天尾静脉注射脂多糖 160μg/kg；阿奇霉素+脂多糖组，妊娠第 9、10、11 天口服阿奇霉素 350mg/kg，第 10 天尾静脉注射脂多糖 160μg/kg。实验第 10 天从尾静脉取血，计算妊娠率。采用 ELISA 法检测肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）水平。阿奇霉素可预防脂多糖诱导的妊娠丢失（P<0.05）。脂多糖组和阿奇霉素+脂多糖组 TNF-α和 IL-10 水平均升高（P<0.05）。综上所述，阿奇霉素可能对感染或内毒素血症相关的妊娠丢失有治疗作用。
