乳腺癌转基因模型的验证：导管原位癌（DCIS）和Brca1突变相关乳腺癌。
Validation of transgenic models of breast cancer: ductal carcinoma in situ (DCIS) and Brca1-mutation-related breast cancer.
作者信息
Frech M S, Jones L P, Furth P A
机构信息
Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University, Washington, DC, USA.
出版信息
Breast Cancer Online. 2005 Aug 1;8(8). doi: 10.1017/S1470903105003135.
Available mouse models of ductal carcinoma (DCIS) and BRCA1-mutation-related breast cancer are reviewed. The best validated mouse models of human DCIS are the conditional estrogen receptor α in mammary tissue (CERM) model initiated by deregulated estrogen receptor α and the serial explant mouse model initiated by p53 deficiency. At present the most useful and best validated mouse model of BRCA1-mutation-related breast cancer uses the cre-lox system to make a conditional Brca1 deletion targeted to mammary epithelial cells. The major shortcoming of the non-conditional Brca1 models is the high incidence of non-mammary tumor development. The use of mammary gland transplants or explants from these mice into nude hosts is one approach that could be used to circumvent this deficiency. Development and validation of a Brca1-mutation-related mouse model of basal cell breast cancer is an important next step.
本文综述了现有的导管原位癌（DCIS）和BRCA1突变相关乳腺癌的小鼠模型。目前，经过充分验证的人类DCIS小鼠模型是由雌激素受体α失调引发的乳腺组织条件性雌激素受体α（CERM）模型，以及由p53缺失引发的连续外植体小鼠模型。目前，BRCA1突变相关乳腺癌最有用且经过充分验证的小鼠模型是利用cre-lox系统，使乳腺上皮细胞发生条件性Brca1缺失。非条件性Brca1模型的主要缺点是乳腺外肿瘤发生的发生率较高。将这些小鼠的乳腺移植或外植体移植到裸鼠宿主中是一种可以用来规避这一缺陷的方法。开发和验证基底细胞乳腺癌的BRCA1突变相关小鼠模型是重要的下一步。
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