Cocchiara R, Di Trapani G, Azzolina A, Albeggiani G, Geraci D
Hum Reprod. 1986 Nov;1(7):445-7. doi: 10.1093/oxfordjournals.humrep.a136452.
In this paper are presented preliminary results on the identification of a histamine-releasing factor. This factor was partially purified by means of affinity heparin-Sepharose chromatography from media in which 2-cell human embryos were cultured. The culture was performed in preparation for an in-vitro fertilization (IVF) programme. The mean value of histamine release evoked by the embryo-derived histamine-releasing factor (EHRF) was 56.7%. The release was not due to cytotoxicity since no histamine release was obtained with unsensitized cells and when the assay was performed at 4 degrees C. As a control, no histamine release was obtained using medium from unfertilized oocytes or medium alone. The EHRF could be one of the first signals from the embryo to the uterus. The immunosuppressive activity of histamine is well known, and we suggest that the local secretion of histamine in vivo by uterine mast cells, alone or in cooperation with other factors and/or mechanisms could play a role in preventing maternal immuno-rejection at the implantation stage.
本文展示了关于组胺释放因子鉴定的初步结果。该因子通过亲和肝素-琼脂糖凝胶色谱法从培养2细胞期人类胚胎的培养基中进行了部分纯化。培养是为体外受精（IVF）程序做准备。胚胎源性组胺释放因子（EHRF）诱发的组胺释放平均值为56.7%。这种释放并非由细胞毒性引起，因为未致敏细胞以及在4℃进行检测时均未出现组胺释放。作为对照，使用未受精卵母细胞的培养基或单独的培养基均未获得组胺释放。EHRF可能是胚胎向子宫发出的首批信号之一。组胺的免疫抑制活性是众所周知的，我们认为子宫肥大细胞在体内局部分泌组胺，单独或与其他因素和/或机制协同作用，可能在防止母体在着床阶段发生免疫排斥中发挥作用。
