Experimental Teaching Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Department of Otolaryngology-Head & Neck Surgery, Shanghai Yueyang Hospital, Shanghai 200437, China.
Evid Based Complement Alternat Med. 2013;2013:762092. doi: 10.1155/2013/762092. Epub 2013 Nov 25.
Objective. To investigate the correlation between presbycusis and kidney deficiency as defined by traditional Chinese medicine (TCM) and its material basis from the perspective of metabolism. Methods. Pure-tone audiometry was used to test auditory function. A kidney deficiency symptom scoring table was used to measure the kidney deficiency accumulated scores of the research subjects. Gas chromatography/mass spectrometry (GC/MS) was used to measure the metabolites in the urine samples from 11 presbycusis patients and 9 elderly people with normal hearing. Results. Hearing loss in the elderly was positively correlated with kidney deficiency score in TCM. There were significant differences in urine metabolite profile between the presbycusis patients and the controls. A total of 23 differentially expressed metabolites were found. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these metabolites were related to glutathione metabolism, amino acid metabolism, glucose metabolism, the N-methyl-D-aspartic acid (NMDA) receptor pathway, and the γ -aminobutyric acid (GABA) receptor pathway. Conclusion. Glutathione metabolism, amino acid metabolism, glucose metabolism, NMDA receptors, and GABA receptors may be related to the pathogenesis of presbycusis and may be the material basis underlying the correlation between presbycusis and kidney deficiency in TCM.
目的。从代谢的角度探讨中医肾虚与老年聋的相关性及其物质基础。方法。采用纯音测听法检测听觉功能，肾虚症状积分表测量研究对象的肾虚累积积分。采用气相色谱/质谱（GC/MS）法检测 11 例老年聋患者和 9 例听力正常老年人的尿液样本中的代谢物。结果。老年人听力损失与中医肾虚评分呈正相关。老年聋患者与对照组的尿液代谢物谱存在显著差异。共发现 23 个差异表达代谢物。京都基因与基因组百科全书（KEGG）通路分析显示，这些代谢物与谷胱甘肽代谢、氨基酸代谢、葡萄糖代谢、N-甲基-D-天冬氨酸（NMDA）受体通路和γ-氨基丁酸（GABA）受体通路有关。结论。谷胱甘肽代谢、氨基酸代谢、葡萄糖代谢、NMDA 受体和 GABA 受体可能与老年聋的发病机制有关，可能是中医肾虚与老年聋相关性的物质基础。
