Lindner G, Grosse G, Jentzsch K D, Oehme P, Wenzel M
J Hirnforsch. 1986;27(6):639-49.
Explants and cells from the peripheral (PNS) and central nervous system (CNS) were cultivated in MAXIMOW chambers. The following four derivatives of Substances P (SP) dipeptide were tested for their effects in concentrations between 10(-4) and 10(-8) mol/l: Lys-Pro X 2HBr, Lys(Z)-Pro X HCl, Lys[Z(NO2)]-Pro X HCl and cyclo(Lys-Pro) X HCl. The results suggest that SP and SP derivatives may modulate nerve fibre outgrowth in vitro. In ganglion trigeminale explants Lys(Z)-Pro X HCl stimulated the growth of nerve fibres. The explant covered areas increased significantly. In telencephalon explants and cell cultures the tested derivatives did not essentially promote neurite outgrowth. In retinal cell cultures incubated with cyclo(Lys-Pro) X HCl and Lys[Z(NO2)]-Pro X HCl the neurite outgrowth index was stimulated in a different way. The neurite length was affected and stimulated in the presence of Lys(Z)-Pro X HCl and cyclo(Lys-Pro) X HCl. Both for survival and differentiation PNS neurons depend on specific biofactors. In sensory ganglia explants SP and especially SP derivatives were shown to improve neuronal survival provided that serum or embryonic extract was simultaneously present in the medium. Even if the dipeptides need other biofactors for their full function the may be discussed as neuronotrophic factors because they ensure survival and general growth capability of the responsive neuroblasts.
取自外周神经系统（PNS）和中枢神经系统（CNS）的外植体及细胞在马克西莫夫培养箱中培养。测试了以下四种P物质（SP）二肽衍生物在浓度为10⁻⁴至10⁻⁸mol/l时的作用：Lys-Pro·2HBr、Lys(Z)-Pro·HCl、Lys[Z(NO₂)]-Pro·HCl和环(Lys-Pro)·HCl。结果表明，SP及其衍生物可能在体外调节神经纤维的生长。在三叉神经节外植体中，Lys(Z)-Pro·HCl刺激了神经纤维的生长。外植体覆盖面积显著增加。在端脑外植体和细胞培养物中，测试的衍生物基本上没有促进神经突的生长。在用环(Lys-Pro)·HCl和Lys[Z(NO₂)]-Pro·HCl孵育的视网膜细胞培养物中，神经突生长指数受到不同方式的刺激。在Lys(Z)-Pro·HCl和环(Lys-Pro)·HCl存在的情况下，神经突长度受到影响并被刺激。PNS神经元的存活和分化都依赖于特定的生物因子。在感觉神经节外植体中，SP尤其是SP衍生物被证明可以提高神经元的存活率，前提是培养基中同时存在血清或胚胎提取物。即使二肽需要其他生物因子才能发挥其全部功能，它们也可以被视为神经营养因子，因为它们确保了反应性神经母细胞的存活和一般生长能力。
