Lindner G, Grosse G, Oehme P, Jentzsch K D, Neubert K
Z Mikrosk Anat Forsch. 1982;96(4):643-55.
Explants of the ganglion trigeminale (PNS) and of the telencephalon (CNS) from chick embryos were cultivated in MAXIMOW chambers in semisynthetic media in the presence of dipeptide fragments (Lys(Z)-Pro . HCl, Lys-Pro-2HBr, Arg-Pro-2HCl) and the heptapeptide (SP5-11) of substance P as well as the complete substance P (SP1-11). 1. Histological examination of the dipeptide-treated CNS explants indicates that the structure of outgrowth in vitro is changed. Fascicel were observed. A stimulation of nerve fibre extension did not take place. 2.1. In dipeptide-treated PNS cultures the index of areas covered by the explants increased. 2.2. The index of nerve fibre growth increased significantly. The stimulation was caused in multiplication of fibres. Only Lys(Z)-Pro . HCl presents a prolongation of neurites. 2.3. SP5-11 effects in no case the growth of nerve fibres. SP1-11 stimulated significantly the fibre regeneration. 3. The possible role of SP1-11 with different effects under in vitro conditions is discussed. Only the N-terminal dipeptides stimulate the growth of nerve fibres. The C-terminal SP5-11 is without effect. Finally it is stated that the best results in neuritic enlargement and neurogenesis can only be obtained by cultivation with SP1-11.
将来自鸡胚的三叉神经节（外周神经系统）和端脑（中枢神经系统）外植体置于MAXIMOW培养室中，在半合成培养基中培养，培养基中存在二肽片段（Lys(Z)-Pro·HCl、Lys-Pro-2HBr、Arg-Pro-2HCl）、P物质的七肽（SP5-11）以及完整的P物质（SP1-11）。1. 对经二肽处理的中枢神经系统外植体进行组织学检查表明，体外生长结构发生了变化。观察到了束状结构。未发生神经纤维延伸的刺激。2.1. 在经二肽处理的外周神经系统培养物中，外植体覆盖面积指数增加。2.2. 神经纤维生长指数显著增加。这种刺激是由纤维增殖引起的。只有Lys(Z)-Pro·HCl能使神经突延长。2.3. SP5-11在任何情况下都不影响神经纤维的生长。SP1-11显著刺激纤维再生。3. 讨论了SP1-11在体外条件下产生不同作用的可能作用。只有N端二肽能刺激神经纤维生长。C端的SP5-11没有作用。最后指出，只有用SP1-11培养才能在神经突增大和神经发生方面获得最佳结果。
