The Department of Hepatobiliary Surgery, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Parasite Immunol. 2014 Apr;36(4):170-6. doi: 10.1111/pim.12096.
The patients with false immune diagnosis of hydatid disease were investigated for the humoural immune response to analyse the possible reasons and mechanism leading to false immune diagnosis. Two hundred and thirty-nine patients with nature-unknown cysts and 30 healthy controls were detected by immunological assays (four hydatid antigen-based immunogold filtration assay and enzyme-linked immune absorbent assay) and ultrasound. Sensitivity of and specificity of immunological assay and ultrasound were calculated, respectively. The serological diagnosis was compared with surgical pathology to screen the patients with false immune diagnosis for the immunoglobulin measurement and pathological analysis. The history and cyst characteristics were also reviewed. The results indicate the immunoglobulin has little influence on false immunodiagnosis. The false-negative immunodiagnosis was caused by the cysts' inactive status while the false positive caused by previous rupture, antigen cross-reaction. The clinical diagnosis of cystic echinococcosis requires a combination of immunodiagnosis and ultrasonography, which is the necessary complementary confirmation.
对假性包虫病患者的体液免疫反应进行了研究，以分析导致假性免疫诊断的可能原因和机制。采用免疫金渗滤试验和酶联免疫吸附试验等 4 种包虫抗原检测试剂盒，对 239 例原因不明的囊肿患者和 30 例健康对照者进行检测，并结合超声检查。计算免疫试验和超声检查的敏感性和特异性，将血清学诊断与手术病理学进行比较，筛选出假性免疫诊断患者，进行免疫球蛋白测量和病理分析。同时回顾病史和囊肿特征。结果表明，免疫球蛋白对假性免疫诊断的影响较小。假阴性免疫诊断是由囊肿的不活动状态引起的，而假阳性是由先前的破裂、抗原交叉反应引起的。囊性包虫病的临床诊断需要免疫诊断和超声检查相结合，这是必要的互补确认。
