Sjölander A, Guisantes J A, Torres-Rodriguez J M, Schröder H
Pharmacia Diagnostics AB, Uppsala, Sweden.
Scand J Infect Dis. 1989;21(2):213-8. doi: 10.3109/00365548909039971.
The sensitivity and specificity of an enzyme immunosorbent assay (EIA) for measurement of anti-Echinococcus granulosus specific IgE antibody using microtiter strips was evaluated. The sensitivity was 92.2% for 90 patients with cysts in the liver or other body sites but excluding the patients with cysts in the lungs or bones. In this latter group (n = 26) a sensitivity of 61.5% was recorded. The specificity for control groups comprising 89 blood bank sera from Spain and 48 sera from atopic Swedes was 100%. Patients infected with other parasites (n = 78) were usually negative (81%). 11 of the 15 false positives were found in patients with total IgE greater than 5,000 kU/l. The microtiter EIA method employed can be considered as a very convenient method to be included in the range of immunodiagnostic tests for human hydatid disease.
评估了使用微量滴定条通过酶免疫吸附测定（EIA）测量抗细粒棘球绦虫特异性IgE抗体的敏感性和特异性。对于90例肝脏或身体其他部位有囊肿但肺部或骨骼无囊肿的患者，敏感性为92.2%。在后一组（n = 26）中，记录的敏感性为61.5%。由89份来自西班牙的血库血清和48份来自特应性瑞典人的血清组成的对照组的特异性为100%。感染其他寄生虫的患者（n = 78）通常为阴性（81%）。15例假阳性患者中有11例总IgE大于5000 kU/l。所采用的微量滴定EIA方法可被认为是一种非常方便的方法，可纳入人类包虫病免疫诊断测试范围。
