Programa de Pos-graduacao em Ciencias Biologicas do Nucleo de Pesquisas em Ciencias Biologicas - NUPEB; Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, 35.400-000, Brasil.
Curr Pharm Biotechnol. 2013;14(11):975-84. doi: 10.2174/1389201014666131226151728.
Acetaminophen is a common analgesic and antipyretic compound which, when administered in high doses, has been associated with significant morbidity and mortality, secondary to hepatic toxicity. Although this may be due to a direct interaction of reactive acetaminophen metabolites with hepatocyte proteins, recent studies have suggested that reactive species produced by neutrophils also contribute to the pathophysiological process. Researches on the chemical composition of B. trimera show that this plant has bioactive compounds such as flavonoids, related to the organism's protection against free radicals. Therefore, in the present study, using Fischer rats, the effect of B. trimera on the antioxidant defense system, the production of nitric oxide (NO) and on the expression of nitric oxide synthase (iNOS), superoxide dismutase (SOD), catalase (CAT) and of the subunits of the NADPH oxidase in neutrophils was evaluated in a model of phagocytosis induced by zimosan (ZC3b) and in a model of inflammation induced by acetaminophen. The results show that the treatment with B. trimera improves the defense system of antioxidant and restores the balance ROS / NO that is altered in the inflammatory process induced by APAP. In conclusion, B. trimera extracts exert antioxidant properties by scavenging ROS and decrease the expression of genes responsible by reactive species production in neutrophils.
对乙酰氨基酚是一种常见的镇痛解热化合物，大剂量使用时可导致严重的发病率和死亡率，其原因是肝毒性。尽管这可能是由于反应性对乙酰氨基酚代谢物与肝细胞蛋白的直接相互作用所致，但最近的研究表明，中性粒细胞产生的反应性物质也参与了病理生理过程。对 B. trimera 的化学成分的研究表明，这种植物具有生物活性化合物，如类黄酮，与机体对抗自由基有关。因此，在本研究中，使用 Fischer 大鼠，评估了 B. trimera 在佐菌素（ZC3b）诱导的吞噬作用模型和对乙酰氨基酚诱导的炎症模型中对抗氧化防御系统、一氧化氮（NO）的产生以及中性粒细胞中一氧化氮合酶（iNOS）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和 NADPH 氧化酶亚基表达的影响。结果表明，B. trimera 的治疗改善了抗氧化防御系统并恢复了 APAP 诱导的炎症过程中改变的 ROS/NO 平衡。总之，B. trimera 提取物通过清除 ROS 发挥抗氧化作用，并降低中性粒细胞中负责产生反应性物质的基因表达。
